About   Help   FAQ
Igs2tm1(CAG-cas9*)Mmw
Targeted Allele Detail
Summary
Symbol: Igs2tm1(CAG-cas9*)Mmw
Name: intergenic site 2; targeted mutation 1, Monte Winslow
MGI ID: MGI:5640626
Synonyms: H11LSL-Cas9
Gene: Igs2  Location: unknown  Genetic Position: Chr11, Syntenic
Alliance: Igs2tm1(CAG-cas9*)Mmw page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:222466
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, Endonuclease, No functional change)
Mutation:    Insertion
 
Mutation detailsThe targeting construct contains a full-length attB site, the CMV enhancer/chicken beta-actin core promoter (CAGGS), a loxP-STOP(3xSV40 polyA)- loxP cassette (LSL), a 3xFLAG sequence, a nuclear localization signal (NLS), a human codon-optimized S. pyogenes cas9 gene (hSpCas9), a second NLS and the SV40 polyA signal. The embryonic stem cells used were previously targeted with the H11 allele ( Igs2tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted insertion of pattB-LSL-Cas9 into the first and second attP sites of the H11 locus. The resulting H11 knock-in allele is therefore attL-CAGGS-LSL-3xFLAG-NLS-hSpCas9-NLS-polyA-attR-attP-FRT5. (J:101977, J:222466)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Igs2 Mutation:  68 strains or lines available
References
Original:  J:222466 Chiou SH, et al., Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory