Reporter:	lacZ
	Direct Detection
Assay notes:	This data was shown in Table 2.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Absent
TS20: stomach epithelium	Absent
TS20: stomach mesenchyme	Present			Medium expression in the forestomach/lower esophageal sphincter; high expression in antrum, corpus or pylorus area.
TS20: small intestine	Present	Regionally restricted		Medium or strong expression in the mesenchyme. Medium expression in the circular muscle layer.
TS20: intestine mesenchyme	Present			Medium or strong expression in the small and large intestine.
TS20: stomach pyloric region mesenchyme	Strong

Structure	Level	Pattern	Image	Note
TS21: esophagus	Absent
TS21: intestine epithelium	Absent
TS21: stomach epithelium	Absent
TS21: stomach pyloric region epithelium	Absent
TS21: stomach mesenchyme	Present	Regionally restricted		Faint expression in the circular muscle layer in the forestomach/lower esophageal sphincter; high expression in both the circular and longitudinal muscle layers and faint expression in lamina propria in some specimens in antrum, corpus or pylorus area.
TS21: stomach pyloric region mesenchyme	Present	Regionally restricted		High expression in both the circular and longitudinal muscle layers. No expression in other mesenchyme. Faint expression in lamina propria in some specimens.
TS21: intestine mesenchyme	Present	Regionally restricted		Faint expression in small intestine mesenchyme in some specimens. Medium to dense expression in large intestine mesenchyme.
TS21: intestine muscularis	Present	Regionally restricted		Dense expression in the circular muscle, no expression in the muscularis externa and the longitudinal muscle in small intestine. Medium expression in muscularis externa, but no expression in circular and longitudinal muscle in large intestine.

Structure	Level	Pattern	Image	Note
TS23: esophagus epithelium	Weak
TS23: stomach mesenchyme	Present	Regionally restricted		Medium expression in the circular muscle layer in the forestomach/lower esophageal sphincter; high expression in both the circular and longitudinal muscle layers and faint expression in lamina propria in some specimens in antrum, corpus or pylorus area.
TS23: intestine muscularis	Present	Regionally restricted		Dense expression in the circular muscle, no expression in the muscularis externa and the longitudinal muscle in small intestine. Medium expression in circular and longitudinal muscle in large intestine.

Structure	Level	Pattern	Image	Note
TS25: esophagus epithelium	Moderate
TS25: esophagus mesenchyme	Absent
TS25: stomach epithelium	Absent
TS25: stomach mesenchyme	Present	Regionally restricted		Medium expression in the circular muscle layer in the forestomach/lower esophageal sphincter; high expression in both the circular and longitudinal muscle layers and faint expression in lamina propria in some specimens in antrum, corpus or pylorus area.
TS25: intestine muscularis	Present	Regionally restricted		Medium expression in the circular muscle, no expression in the muscularis externa and the longitudinal muscle in small intestine. Medium to dense expression in circular and longitudinal muscle in large intestine.

Structure	Level	Pattern	Image	Note
TS28: esophagus epithelium	Strong
TS28: stomach smooth muscle	Moderate	Regionally restricted		Medium expression in the circular muscle layer in the forestomach/lower esophageal sphincter; medium expression in both the circular and longitudinal muscle layers and lamina propria in antrum, corpus or pylorus area.
TS28: small intestine	Absent
TS28: cecum	Moderate	Regionally restricted		Faint to medium expression in the circular muscle.
TS28: large intestine smooth muscle	Moderate	Regionally restricted		Expressed in the circular muscle.