Gene Expression Data
Assay Details
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Assay
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Reference:
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J:162172
Fox EA, et al., Smooth-muscle-specific expression of neurotrophin-3 in mouse embryonic and neonatal gastrointestinal tract. Cell Tissue Res. 2010 May;340(2):267-86
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5638237
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Gene symbol:
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Ntf3
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Gene name:
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neurotrophin 3
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
1e:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more  )
Specimen
1e:
(close  )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2a:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
2a:
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: stomach mesenchyme |
Present |
Regionally restricted |
2a
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Expressed in the smooth muscle layers. Weak staining in the lamina propria of the stomach antmm and corpus.
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| TS25: blood vessel |
Present |
Regionally restricted |
2a
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Expressed in the smooth muscle layers.
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Specimen
2b:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
2b:
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: small intestine |
Present |
Regionally restricted |
2b
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Expressed in the smooth muscle layers.
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Specimen
2c:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
2c:
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: large intestine |
Present |
Regionally restricted |
2c
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Expressed in the smooth muscle layers.
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Specimen
12b/b":
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: red - Ntf3 reporter; green - Ngfr (assay MGI:5638392).
Specimen
12b/b":
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntf3 reporter; green - Ngfr (assay MGI:5638392).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: small intestine |
Present |
Regionally restricted |
12b"
,
12b
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Not expressed in undifferentiated neural crest cells.
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Specimen
12c/c":
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: red - Ntf3 reporter; green - S100b (assay MGI:5638393).
Specimen
12c/c":
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntf3 reporter; green - S100b (assay MGI:5638393).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: large intestine |
Present |
Regionally restricted |
12c
,
12c"
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Not expressed in glial cells.
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Specimen
12d/d":
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: red - Ntf3 reporter; green - Kit (assay MGI:5638394).
Specimen
12d/d":
(close )
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Genetic Background:
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B6.129S2(Cg)-Ntf3tm1Lfr
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Ntf3 reporter; green - Kit (assay MGI:5638394).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: stomach |
Present |
Regionally restricted |
12d
,
12d"
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Not expressed in interstitial cells of Cajal.
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