Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(ACTA1-PRKAG1*R70Q)3Lwit
Name:	transgene insertion 3, Lee Witters
MGI ID:	MGI:5637561
Synonyms:	HA-R70Qgamma1
Transgene:	Tg(ACTA1-PRKAG1*R70Q)3Lwit  Location: unknown
Alliance:	Tg(ACTA1-PRKAG1*R70Q)3Lwit page

Strain of Origin:

FVB/NJ

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence) Mutations:    Insertion, Nucleotide substitutions   Tg(ACTA1-PRKAG1*R70Q)3Lwit expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human PRKAG1 (5571) Prkag1  (MGI:108411) The cDNA carries mutation R70Q.   Mutation details: The 3.6 kbp transgene contains a human skeletal muscle alpha-actin promoter (~2.2 kbp of the 5prime region of ACTA1), a full-length human AMP-activated protein kinase non-catalytic gamma1 (PRKAG1) cDNA sequence harboring the R70Q amino acid substitution tagged to the 5' end with a hemagglutinin (HA) epitope, all followed by the SV40 sequence. Immunoblot detects transgene expression in gastrocnemius, tibialis anterior, quadriceps and extensor digitorum longus muscles. There is little detectable expression in the soleus and low level expression in heart. Founder line 3 has approximately threefold increase in total gamma1 gene copy number compared with wildtype mice

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:118883 Barre L, et al., Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation. Am J Physiol Endocrinol Metab. 2007 Mar;292(3):E802-11
All:	3 reference(s)