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Gene Expression Data
Assay Details
Assay
Reference: J:112483 Thompson DL, et al., Ontogeny of Phex/PHEX protein expression in mouse embryo and subcellular localization in osteoblasts. J Bone Miner Res. 2002 Feb;17(2):311-20
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5633804
Gene symbol: Phex
Gene name: phosphate regulating endopeptidase homolog, X-linked
Antibody: PHEX antibody
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Fixative was 4% formaldehyde.
Results
Specimen 1A: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: lumbar vertebral cartilage condensation Absent 1A Absent in the cells of primary centers of ossification in thoracolumbar and lumbar vertebrae.
Specimen 1B: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: vertebra Present Regionally restricted 1B Expression is in osteoblasts within primary ossifying centers, absent in chondrocytes.
Specimen not shown: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: limb skeleton Present Regionally restricted Expression is in ossification centers in the limbs.
Specimen 1C: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: vertebra Present Regionally restricted 1C Expression is in osteoblasts in primary ossification regions.
Specimen 1E: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: chondrocranium Strong Regionally restricted 1E Expression is in osteoblasts.
TS26: epidermis granular layer Strong 1E
TS26: epidermis spinous layer Strong 1E
Specimen 1F: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: chondrocranium Moderate Regionally restricted 1F Expression is in osteoblasts and osteocytes.
Specimen not shown P w 4: postnatal week 4 (more )
Structure Level Pattern Image Note
TS28: chondrocranium Very strong Regionally restricted Expression is in osteocytes in the compact bone.
Specimen 2A: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: tibia Moderate Regionally restricted 2A Expression is in osteoblasts and to a lesser degree osteocytes of both the secondary and the primary centers of ossification. Absent in chondrocytes in the zones of the epiphyseal plate.
Specimen 2B: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: tibia Moderate Regionally restricted 2B Expression is in osteoblasts and to a lesser degree osteocytes of both the secondary and the primary centers of ossification.
Specimen 2D: postnatal week 12 (more )
Structure Level Pattern Image Note
TS28: tibia Very strong Regionally restricted 2D Expression is in osteocytes of the compact bone.
Specimen 2E: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: incisor dental pulp Very strong Regionally restricted 2E Expression is in odontoblasts.
TS28: incisor enamel organ Present Regionally restricted 2E Expression is in ameloblasts.
Specimen 2F: postnatal week 12 (more )
Structure Level Pattern Image Note
TS28: incisor dental pulp Present Regionally restricted 2F Expression is in odontoblasts.
TS28: incisor enamel organ Trace Regionally restricted 2F Faint to absent staining in ameloblasts.
Specimen 6A: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: maturing glomerular tuft Absent 6A
TS26: renal tubule Absent 6A
Specimen 6B: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: intestine Absent 6B
Specimen 6C: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: skeletal muscle Absent 6C
Specimen 6D: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: liver Moderate Single cells 6D Staining is in macrophages within the liver.
Specimen 6E: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: cardiac muscle tissue Absent 6E
Specimen 6F: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: brown fat Absent 6F
Specimen not shown E18: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: brain Absent
TS26: lung Absent
Specimen 7A: postnatal newborn; PhexHyp/? (more )
Note: the sex of the specimen was not reported; the mutation is on the X chromosome
Structure Level Pattern Image Note
TS27: chondrocranium Absent 7A
TS27: skin Absent 7A
Specimen 7B: postnatal newborn; PhexHyp/? (more )
Note: the sex of the specimen was not reported; the mutation is on the X chromosome
Structure Level Pattern Image Note
TS27: vertebra Absent 7B Absent in mesenchymal cells and osteoblasts.
Specimen 7C: postnatal newborn; PhexHyp/? (more )
Note: the sex of the specimen was not reported; the mutation is on the X chromosome
Structure Level Pattern Image Note
TS27: vertebra Absent 7C Absent in osteoblasts.
Specimen 7D: postnatal newborn; PhexHyp/? (more )
Note: the sex of the specimen was not reported; the mutation is on the X chromosome
Structure Level Pattern Image Note
TS27: brown fat Absent 7D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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