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Gene Expression Data
Assay Details
Assay
Reference: J:204010 Garner JL, et al., Stability of DNA methylation patterns in mouse spermatogonia under conditions of MTHFR deficiency and methionine supplementation. Biol Reprod. 2013 Nov;89(5):125
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5633790
Gene symbol: Mthfr
Gene name: methylenetetrahydrofolate reductase
Antibody: MTHFR antibody
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Specimens were either fixed by perfusion through the heart with 50% Sainte-Marie fixative or testes were removed and fixed by immersion in Bouin's solution.
Results
Specimen 2A: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: testis Absent 2A
Specimen 2B: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2B Expression is in the cytoplasm of prospermatogonia.
Specimen 2C: embryonic day 18.0 (more )
Structure Cell Type Level Pattern Image Note
TS26: germ cell of testis Present Regionally restricted 2C Expression is in the cytoplasm of prospermatogonia.
TS26: Sertoli cell Sertoli cell Present Diffuse 2C
TS26: interstitium of the testis Present Single cells 2C Expression is in some interstitial cells.
Specimen 2D: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: spermatogonium Very strong 2D Expression is in prospermatogonia.
TS27: Sertoli cell Sertoli cell Present 2D
Specimen 2E: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: spermatogonium Very strong 2E Expression is in prospermatogonia.
TS27: Sertoli cell Sertoli cell Present 2E
Specimen 3A: postnatal week 8-10 (more )
Note: Stage VII of the cycle of the seminiferous epithelium.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 3A
TS28: primary spermatocyte Present 3A Expression is in preleptotene and pachytene spermatocytes.
TS28: spermatid Present 3A Expression is in early and late spermatids.
TS28: interstitium of the testis Absent 3A
Specimen 3B: postnatal week 8-10 (more )
Note: Stage XII of the cycle of the seminiferous epithelium.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 3B
TS28: spermatocyte Present Single cells 3B The meiotic divisions of spermatocytes stain positive, absent in zygotene spermatocytes.
TS28: spermatid Present 3B Expression is in elongating step 12 spermatids.
TS28: interstitium of the testis Absent 3B
Specimen 3C: postnatal week 8-10 (more )
Note: Stage XI of the cycle of the seminiferous epithelium.
Structure Level Pattern Image Note
TS28: spermatocyte Present Single cells 3C Expression is in type A spermatogonia at the base of the seminiferous epithelium and is absent in zygotene spermatocytes.
Specimen 3D: postnatal week 8-10; Mthfrtm1Rzn/Mthfrtm1Rzn (more )
Structure Level Pattern Image Note
TS28: testis Absent 3D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory