Antibody:	MTHFR antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Specimens were either fixed by perfusion through the heart with 50% Sainte-Marie fixative or testes were removed and fixed by immersion in Bouin's solution.

Structure	Level	Pattern	Image	Note
TS21: testis	Absent		2A

Structure	Level	Pattern	Image	Note
TS23: germ cell of testis	Present		2B	Expression is in the cytoplasm of prospermatogonia.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: germ cell of testis		Present	Regionally restricted	2C	Expression is in the cytoplasm of prospermatogonia.
TS26: Sertoli cell	Sertoli cell	Present	Diffuse	2C
TS26: interstitium of the testis		Present	Single cells	2C	Expression is in some interstitial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: spermatogonium		Very strong		2D	Expression is in prospermatogonia.
TS27: Sertoli cell	Sertoli cell	Present		2D

Structure	Cell Type	Level	Pattern	Image	Note
TS27: spermatogonium		Very strong		2E	Expression is in prospermatogonia.
TS27: Sertoli cell	Sertoli cell	Present		2E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		3A
TS28: primary spermatocyte		Present		3A	Expression is in preleptotene and pachytene spermatocytes.
TS28: spermatid		Present		3A	Expression is in early and late spermatids.
TS28: interstitium of the testis		Absent		3A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		3B
TS28: spermatocyte		Present	Single cells	3B	The meiotic divisions of spermatocytes stain positive, absent in zygotene spermatocytes.
TS28: spermatid		Present		3B	Expression is in elongating step 12 spermatids.
TS28: interstitium of the testis		Absent		3B

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Single cells	3C	Expression is in type A spermatogonia at the base of the seminiferous epithelium and is absent in zygotene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		3D