Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36691
Peters JH, et al., Fibronectin isoform distribution in the mouse. I. The alternatively spliced EIIIB, EIIIA, and V segments show widespread codistribution in the developing mouse embryo. Cell Adhes Commun. 1996 Aug;4(2):103-25
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5631987
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Gene symbol:
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Fn1
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Gene name:
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fibronectin 1
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Antibody:
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anti-EIIIB FN
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Data is summarized in Table II. Most of the data refer to the maximum overall staining intensity regardless of the embryonic timepoint. Only the data stated as E16.5 was annotated. Staining intensity was subjectively graded as (-) negative, (+) faint, (++) moderate, and (+++) strong. Staining intensity was subjectively graded as (-) negative, (+) faint, (++) moderate, and (+++) strong.
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Results
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Specimen
PARAMESONEPHRIC DUCTS:
embryonic day 16.5
(more  )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
PARAMESONEPHRIC DUCTS:
(close  )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: paramesonephric duct |
Strong |
Regionally restricted |
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Expressed in the basement membrane zone and the subepithelial connective tissues.
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Specimen
TESTIS:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
TESTIS:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: testis mesenchyme |
Strong |
Regionally restricted |
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Expressed in the basement membrane zone and the mesothelial.
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Specimen
ELASTIC CARTILAGE:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
ELASTIC CARTILAGE:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cartilage |
Strong |
Regionally restricted |
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Expressed in the elastic cartilage.
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Specimen
SYNOVIUM:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
SYNOVIUM:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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Specimen
SPLEEN:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
SPLEEN:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Strong |
Regionally restricted |
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Expressed in capillary and small blood vessels.
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| TS24: spleen |
Strong |
Regionally restricted |
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Expressed in capillary and small blood vessels, and the subepithelial connective tissue.
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Specimen
THYMUS:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
THYMUS:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Strong |
Regionally restricted |
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Expressed in capillary and small blood vessels.
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| TS24: thymus |
Strong |
Regionally restricted |
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Expressed in capillary and small blood vessels.
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Specimen
THYROID:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
THYROID:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thyroid gland |
Moderate |
Regionally restricted |
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Expressed in the basement membrane zone and the subepithelial connective tissues.
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Specimen
PITUITARY:
embryonic day 16.5
(more )
Note: Fixed in 2.5% paraformaldehyde.
Specimen
PITUITARY:
(close )
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Genetic Background:
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either: CD-1 or FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Fixed in 2.5% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Moderate |
Regionally restricted |
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Expressed in capillary and small blood vessels.
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| TS24: pituitary gland |
Moderate |
Regionally restricted |
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Expressed in capillary and small blood vessels.
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Analysis Tools