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Gene Expression Data
Assay Details
Assay
Reference: J:197857 Ahmedli NB, et al., Dynamics of the rhomboid-like protein RHBDD2 expression in mouse retina and involvement of its human ortholog in retinitis pigmentosa. J Biol Chem. 2013 Apr 5;288(14):9742-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5631980
Gene symbol: Rhbdd2
Gene name: rhomboid domain containing 2
Antibody: 7Rc antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Confocal images.
Results
Specimen 6B: postnatal adult (more )
Note: Double labeled: green - Rhbdd2; red - Golga2 (assay MGI:5631986).
Structure Level Pattern Image Note
TS28: photoreceptor layer inner segment Present 6B 7RC , 6B higher mag top , 6B Merged
TS28: retina ganglion cell layer Present 6B higher mag bottom , 6B Merged , 6B 7RC Expression is perinuclear.
TS28: retina inner nuclear layer Weak 6B 7RC , 6B Merged Expression is at the inner nuclear layer edges bordering the outer plexiform layer and inner plexiform layer.
Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: optic cup Weak
Specimen 7A E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: retina Present Ubiquitous 7A 7Rc/DAPI E12.5 , 7A bottom left , 7A 7Rc E12.5
Specimen 7A E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: retina Present Ubiquitous 7A 7Rc E15.5 , 7A 7Rc/DAPI E15.5
Specimen 7A E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: retina Strong Ubiquitous 7A 7Rc E18.5 , 7A 7Rc/DAPI E18.5
Specimen 7B P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present Single cells 7B 7Rc/DAPI P1 , 7B 7Rc P1
TS27: retina Present Single cells 7B 7Rc P1 , 7B 7Rc/DAPI P1 Expression is in cells at the inner edge of the ventricular zone of the retina.
Specimen 7B P5: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells 7B 7Rc P5 , 7B 7Rc/DAPI P5
TS28: retina Present Single cells 7B 7Rc P5 , 7B 7Rc/DAPI P5 Expression is in cells at the inner edge of the ventricular zone of the retina.
Specimen 7B P8: postnatal day 8 (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 7B 7Rc P8 , 7B 7Rc/DAPI P8
TS28: retina inner nuclear layer Present 7B 7Rc P8 , 7B 7Rc/DAPI P8
TS28: retina outer nuclear layer Trace 7B 7Rc P8 , 7B 7Rc/DAPI P8
Specimen 7B P11: postnatal day 11 (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 7B 7Rc/DAPI P11 , 7B 7Rc P11
TS28: retina inner nuclear layer Present 7B 7Rc/DAPI P11 , 7B 7Rc P11
TS28: retina outer nuclear layer Trace 7B 7Rc/DAPI P11 , 7B 7Rc P11
Specimen 7B P21: postnatal day 21 (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 7B 7Rc/DAPI P21 , 7B 7Rc P21
TS28: retina outer nuclear layer Present 7B 7Rc/DAPI P21 , 7B 7Rc P21 Expression is mainly around the nuclei of photoreceptors.
Specimen 8A: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: retina layer Strong Regionally restricted 8A 7Rc/DAPI , 8A 7Rc Expression is in a small number of irregular distributed cells with short outer segments located in the outer retina.
Specimen 8B: postnatal day 12 (more )
Note: Double labeled: green - Rhbdd2; red - Opn1mw (assay MGI:5631994).
Structure Level Pattern Image Note
TS28: retina layer Present 8B Merged , 8B 7Rc Expression is in a small number of irregular distributed cells with short outer segments located in the outer retina.
Specimen 8C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: photoreceptor layer Present 8C
TS28: retina outer nuclear layer Present 8C
TS28: photoreceptor layer outer segment Present Regionally restricted 8C
Specimen 8D: postnatal adult (more )
Note: Double labeled: green - Rhbdd2; red - Glul (assay MGI:5629832).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Strong 8D Merged , 8D 7Rc
TS28: retina inner nuclear layer Strong 8D 7Rc , 8D Merged Stronger staining in Muller cells.
TS28: retina outer nuclear layer Weak 8D Merged , 8D 7Rc Expression is in the processes of Muller cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory