Antibody:	anti-V95 FN
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1B	Expressed in both punctate intracellular and fibrillar intercellular patterns.
TS11: exocoelomic cavity	Strong	Regionally restricted	1B	Expressed in cells lining the exocoelomic cavity.
TS11: embryo	Strong	Regionally restricted	1B	Expressed in the basement membrane lying between epithelial sheets of ectoderm and endoderm.
TS11: embryo ectoderm	Absent		1B
TS11: amniotic cavity	Absent		1B	No expression in the lining cells.
TS11: ectoplacental cavity	Absent		1B	No expression in the lining cells.
TS11: embryo endoderm	Present	Punctate	1B	Expressed in the apices of cells.
TS11: extraembryonic endoderm	Present	Punctate	1B	Expressed in the apices of cells.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2_V95 Top	Expressed in the surrounding membrane.
TS15: dorsal aorta	Present	Regionally restricted	2_V95 Top	Expressed in the wall.
TS15: sinus venosus	Present		2_V95 Top
TS15: gut	Present	Regionally restricted	2_V95 Top	Expressed in the epithelial basement membrane.
TS15: gut mesenchyme	Present	Regionally restricted	2_V95 Top	Expressed in the subepithelial connective tissue.
TS15: gut epithelium	Absent		2_V95 Top
TS15: pericardial cavity	Absent		2_V95 Top
TS15: yolk sac	Present	Regionally restricted	2_V95 Top	Expressed in the parietal yolk sac.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	2_V95 Middle	Expressed in the periphery.
TS15: neural tube	Present	Regionally restricted	2_V95 Middle	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	2_V95 Bottom	Expressed in the surrounding membrane.
TS17: blood vessel	Present	Regionally restricted	2_V95 Bottom	Expressed in the intersegmental blood vessels.

Structure	Level	Pattern	Image	Note
TS15: yolk sac mesenchyme	Strong		3C	Fibrillar expression in the inner mesoderm-derived layer, including the blood vessels.
TS15: yolk sac endoderm	Absent		3C

Structure	Level	Pattern	Image	Note
TS15: allantois	Present	Regionally restricted	3F	Expressed in the lining cells.
TS15: spongiotrophoblast	Present		3F
TS15: labyrinthine zone	Present	Regionally restricted	3F	Not expressed in the compressed ectoplacental plate.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Diffuse	4V95 Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS17: outflow tract	Strong	Diffuse	4V95 Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Regionally restricted	4V95 Bottom	Expressed in a perimysial/vascular pattern.
TS17: visceral pericardium	Present		4V95 Bottom	Expressed in epicardium.
TS17: endocardial lining	Present		4V95 Bottom	Expressed in endocardium.

Structure	Level	Pattern	Image	Note
TS20: trachea	Present	Regionally restricted	5V95 Top	Expressed in the basement membrane and underlining subepithelial connective tissue.
TS20: esophagus epithelium	Absent		5V95 Top
TS20: nasopharynx epithelium	Absent		5V95 Top
TS20: trachea epithelium	Absent		5V95 Top
TS20: trachea mesenchyme	Present		5V95 Top	Expressed in the connective tissue.
TS20: esophagus	Present	Regionally restricted	5V95 Top	Expressed in the basement membrane and surrounding smooth muscle.
TS20: dorsal aorta	Present	Regionally restricted	5V95 Top	Expressed in the wall as a multilaminar staining.

Structure	Level	Pattern	Image	Note
TS17: trachea	Present	Regionally restricted	5V95 Middle	Expressed in the basement membrane and the surrounding interstitial tissue, including small blood vessels.
TS17: trachea vascular element	Present	Regionally restricted	5V95 Middle	Expressed in small blood vessels.
TS17: trachea epithelium	Absent		5V95 Middle

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	5V95 Bottom	Expressed in the wall.
TS23: main bronchus	Present	Regionally restricted	5V95 Bottom	Expressed in the epithelial basement membrane, with sparing of epithelial cells.
TS23: main bronchus epithelium	Absent		5V95 Bottom

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	6V95 Top row	Expressed in the basement membrane underlying the epithelium, the lamina propria and smooth muscle.
TS20: stomach mesenchyme	Present	Regionally restricted	6V95 Top row	Expressed in smooth muscle.
TS20: pancreatic duct	Strong	Regionally restricted	6V95 Top row	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	6V95 Second row	Expressed in the epithelial basement membrane.
TS20: gut epithelium	Absent		6V95 Second row
TS20: gut mesenchyme	Present	Regionally restricted	6V95 Second row	Expressed in smooth muscle.

Structure	Level	Pattern	Image	Note
TS17: vitelline vein	Strong		6V95 Third row	Expressed in the wall.
TS17: arterial system	Strong	Regionally restricted	6V95 Third row	Expressed in the wall of the mesenteric artery.
TS17: pancreas primordium	Present	Regionally restricted	6V95 Third row	Expressed in the basement membranes of the developing acini and ducts.
TS17: venous system	Strong	Regionally restricted	6V95 Third row	Expressed in the wall of the portal vein.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	6V95 Bottom row	Expressed in a reticular pattern.
TS20: liver vascular element	Present	Regionally restricted	6V95 Bottom row	Expressed in the walls of hepatic veins.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		7V95 Top row

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		7V95 Second row
TS20: mesonephros	Present		7V95 Second row
TS20: vertebral cartilage condensation	Present		7V95 Second row
TS20: notochord	Strong	Regionally restricted	7V95 Second row	Fibrillar expression in the lumen of the cylinder.

Structure	Level	Pattern	Image	Note
TS24: vertebral cartilage condensation	Present	Diffuse	7V95 Third row	Cell and matrix staining.
TS24: nucleus pulposus	Strong	Regionally restricted	7V95 Third row	Fibrillar expression in the lumen of the cylinder of the notochord.

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		7V95 Bottom row
TS24: Meckel's cartilage	Present	Regionally restricted	7V95 Bottom row	Expressed in the peripheral in addition to diffuse matrix staining.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	8V95 Top row	Expressed in brain blood vessels and basement membrane of the meninges.
TS20: blood vessel	Present	Regionally restricted	8V95 Top row	Expressed in the basement membrane.
TS20: remnant of Rathke's pouch	Present	Regionally restricted	8V95 Top row	Expressed in the basement membrane.
TS20: face mesenchyme	Present	Regionally restricted	8V95 Top row	Expressed in the small blood vessels and cells within the cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	8V95 Second row	Expressed in the epithelial basement membrane.
TS17: 3rd ventricle	Present	Regionally restricted	8V95 Second row	Expressed in the ependyma of the infundibulum. Not expressed in the cels lining the infundibulum.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present	Regionally restricted	8V95 Third row	Expressed in the inner capillary network, and the epithelial basement membrane. Not expressed in the epithelial cells.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted	8V95 Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord	Present	Regionally restricted	8V95 Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord vascular element	Present		8V95 Bottom row

Structure	Level	Pattern	Image	Note
TS28: myometrium	Strong	Regionally restricted		Expressed in the peripheral smooth muscle.
TS28: mesometrium	Strong	Regionally restricted		Expressed in the walls of the dilated blood sinuses.
TS28: decidua	Ambiguous	Regionally restricted		Little or no expression in the tissue surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Regionally restricted		Expressed in the large blood vessels at the periphery.
TS28: myometrium	Present	Regionally restricted		Expressed in the inner circular layer smooth muscle and the dilated blood sinuses.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Strong	Regionally restricted		Expressed in the trophoblast giant cells, but not the ectoplacental cone.
TS15: trophoblast giant cell	Strong
TS28: decidua	Strong	Regionally restricted		Expressed in the walls of vascular structures.
TS15: foregut epithelium	Present
TS15: labyrinthine zone	Present
TS15: spongiotrophoblast	Present	Regionally restricted		Interstitial expression.
TS15: notochord	Present	Regionally restricted		Expressed in the periphery and the surrounding mesenchyme.
TS15: neural tube	Present	Regionally restricted		Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: cartilage condensation	Present
TS17: notochord	Present			Including the sheath.
TS17: eye mesenchyme	Present	Regionally restricted		Expressed in the extraocular muscles. Fibrillar staining in the perioptic mesenchyme, particularly the walls of capillaries, small blood vessels and the hyaloid vescular network.
TS17: paraxial mesenchyme	Present	Regionally restricted		Expressed in paraspinal muscles.
TS17: optic cup inner layer	Absent			No expression in the neural retina.
TS17: lens pit	Present	Regionally restricted		Expressed in the capsule, not the cells lining the vesicle.
TS17: eye surface ectoderm	Absent			Corneal ectodermal layer.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: outflow tract	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: cartilage	Present
TS20: endocardial lining	Present
TS20: mesenchyme of metanephros	Present
TS20: pericardium	Present
TS20: perichondrium	Present
TS20: renal vesicle	Present
TS20: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: metanephros	Present	Regionally restricted		Expressed in the basement membranes of the developing tubules and vesicles.
TS20: ureter	Present	Regionally restricted		Expressed in the walls of the renal pelvis.
TS20: renal capsule	Absent
TS20: retina	Absent
TS20: urogenital ridge	Present	Regionally restricted		Expressed in the mesothelial lining.
TS20: mesonephros	Present	Regionally restricted		Expressed in the interstitium.
TS20: mesonephric tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS20: notochord	Present			Including the sheath.
TS20: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS20: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS20: epidermis	Ambiguous			Relative lack of staining.

Structure	Level	Pattern	Image	Note
TS23: Bowman's capsule of maturing renal corpuscle	Present
TS23: cartilage	Present
TS23: endocardial tissue	Present
TS23: eyelid mesenchyme	Present
TS23: glomerular basement membrane of maturing renal corpuscle	Present
TS23: glomerular mesangium of maturing glomerular tuft	Present
TS23: pericardium	Present
TS23: perichondrium	Present
TS23: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: renal tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS23: renal interstitium	Present	Regionally restricted		Expressed in tubular interstium.
TS23: nucleus pulposus	Strong	Regionally restricted		Expressed in the sheath.
TS23: cornea stroma	Strong	Regionally restricted		Fibrillar staining of the mesothelial layer.
TS23: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS23: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS23: epidermis	Ambiguous			Relative lack of staining.

Structure	Level	Pattern	Image	Note
TS24: Bowman's capsule of maturing renal corpuscle	Present
TS24: cartilage	Present
TS24: endocardial tissue	Present
TS24: lens capsule	Present
TS24: pericardium	Present
TS24: perichondrium	Present
TS24: tunica albuginea of testis	Present
TS24: vasculature of bladder	Present
TS24: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: maturing glomerular tuft	Present	Regionally restricted		Expressed in the glomerular basement membrane, the glomerular mesangium and the walls of glomerular capillaries.
TS24: renal tubule	Present	Regionally restricted		Expressed in the tubular basement membrane and tubular interstium.
TS24: bladder	Present	Regionally restricted		Expressed in the epitheial basement membrane and the subepithelial connective tissue.
TS24: retina	Absent
TS24: urothelium of bladder	Absent
TS24: detrusor muscle of bladder	Present	Regionally restricted		Expressed in the enveloping smooth muscle.
TS24: seminiferous cord	Present	Regionally restricted		Expressed in the basement membrane.
TS24: testis mesenchyme	Present	Regionally restricted		Expressed in the interstitium.
TS24: nucleus pulposus	Present			Including the sheath.
TS24: arytenoid cartilage	Present			Elastic cartilage.
TS24: metatarsus	Present	Regionally restricted		Expressed in the ends of the bones.
TS24: extrinsic ocular muscle	Present	Diffuse		Expressed in reticular patterns.
TS24: axial muscle	Present	Regionally restricted		Reticular expression in the paraspinal muscles.
TS24: spleen	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: thymus	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: adenohypophysis pars anterior	Present			Expressed in the blood vessels, not the parenchyma.
TS24: thyroid gland	Present	Regionally restricted		Expressed in the basement membranes and the vascular interstitium.
TS24: adrenal gland	Present	Regionally restricted		Expressed in the capsule and the interstitium.
TS24: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS24: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS24: epidermis	Ambiguous			Relative lack of staining.