Antibody:	anti-total FN
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1A	Expressed in both punctate intracellular and fibrillar intercellular patterns.
TS11: exocoelomic cavity	Strong	Regionally restricted	1A	Expressed in cells lining the exocoelomic cavity.
TS11: embryo	Strong	Regionally restricted	1A	Expressed in the basement membrane lying between epithelial sheets of ectoderm and endoderm.
TS11: embryo ectoderm	Absent		1A
TS11: amniotic cavity	Absent		1A	No expression in the lining cells.
TS11: ectoplacental cavity	Absent		1A	No expression in the lining cells.
TS11: embryo endoderm	Present	Punctate	1A	Expressed in the apices of cells.
TS11: extraembryonic endoderm	Present	Punctate	1A	Expressed in the apices of cells.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Diffuse	4T Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS17: outflow tract	Strong	Diffuse	4T Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Regionally restricted	4T Bottom	Expressed in a perimysial/vascular pattern.
TS17: visceral pericardium	Present		4T Bottom	Expressed in epicardium.
TS17: endocardial lining	Present		4T Bottom	Expressed in endocardium.

Structure	Level	Pattern	Image	Note
TS20: trachea	Present	Regionally restricted	5T Top	Expressed in the basement membrane and underlining subepithelial connective tissue.
TS20: esophagus epithelium	Absent		5T Top
TS20: trachea epithelium	Absent		5T Top
TS20: trachea mesenchyme	Present		5T Top	Expressed in the connective tissue.
TS20: esophagus	Present	Regionally restricted	5T Top	Expressed in the basement membrane and surrounding smooth muscle.
TS20: dorsal aorta	Present	Regionally restricted	5T Top	Expressed in the wall as a multilaminar staining.

Structure	Level	Pattern	Image	Note
TS17: trachea	Present	Regionally restricted	5T Middle	Expressed in the basement membrane and the surrounding interstitial tissue, including small blood vessels.
TS17: trachea vascular element	Present	Regionally restricted	5T Middle	Expressed in small blood vessels.
TS17: trachea epithelium	Absent		5T Middle

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	5T Bottom	Expressed in the wall.
TS23: main bronchus	Present	Regionally restricted	5T Bottom	Expressed in the epithelial basement membrane, with sparing of epithelial cells.
TS23: main bronchus epithelium	Absent		5T Bottom

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	6T Top row	Expressed in the basement membrane underlying the epithelium, the lamina propria and smooth muscle.
TS20: stomach mesenchyme	Present	Regionally restricted	6T Top row	Expressed in smooth muscle.
TS20: pancreatic duct	Strong	Regionally restricted	6T Top row	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	6T Second row	Expressed in the epithelial basement membrane.
TS20: gut epithelium	Absent		6T Second row
TS20: gut mesenchyme	Present	Regionally restricted	6T Second row	Expressed in smooth muscle.

Structure	Level	Pattern	Image	Note
TS17: vitelline vein	Strong		6T Third row	Expressed in the wall.
TS17: arterial system	Strong	Regionally restricted	6T Third row	Expressed in the wall of the mesenteric artery.
TS17: pancreas primordium	Present	Regionally restricted	6T Third row	Expressed in the basement membranes of the developing acini and ducts.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	6T Bottom row	Expressed in a reticular pattern.
TS20: liver vascular element	Present	Regionally restricted	6T Bottom row	Expressed in the walls of hepatic veins.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	8T Top row	Expressed in brain blood vessels and basement membrane of the meninges.
TS20: blood vessel	Present	Regionally restricted	8T Top row	Expressed in the basement membrane.
TS20: remnant of Rathke's pouch	Present	Regionally restricted	8T Top row	Expressed in the basement membrane.
TS20: face mesenchyme	Present	Regionally restricted	8T Top row	Expressed in the small blood vessels and cells within the cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	8T Second row	Expressed in the epithelial basement membrane.
TS17: 3rd ventricle	Present	Regionally restricted	8T Second row	Expressed in the ependyma of the infundibulum.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present	Regionally restricted	8T Third row	Expressed in the inner capillary network, and the epithelial basement membrane.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted	8T Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord	Present	Regionally restricted	8T Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord vascular element	Present		8T Bottom row

Structure	Level	Pattern	Image	Note
TS28: myometrium	Present	Regionally restricted		Expressed in the peripheral smooth muscle and the walls of the central uterine artery along its spinal course.
TS28: decidua	Present	Widespread		Expressed in decidual interstitium, including the tissue surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Regionally restricted		Expressed in the large blood vessels at the periphery.
TS28: myometrium	Present	Regionally restricted		Expressed in the inner circular layer smooth muscle and the dilated blood sinuses.
TS28: decidua	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Strong	Regionally restricted		Expressed in the trophoblast giant cells, but not the ectoplacental cone.
TS15: trophoblast giant cell	Strong
TS28: decidua	Strong	Regionally restricted		Expressed in the walls of vascular structures.
TS15: labyrinthine zone	Present
TS15: spongiotrophoblast	Present	Regionally restricted		Interstitial expression.
TS15: allantois	Present	Regionally restricted		Expressed in the lining cells.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Absent			No expression in the retina.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: outflow tract	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: endocardial lining	Present
TS20: mesenchyme of metanephros	Present
TS20: pericardium	Present
TS20: renal vesicle	Present
TS20: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: metanephros	Present	Regionally restricted		Expressed in the basement membranes of the developing tubules and vesicles.
TS20: ureter	Present	Regionally restricted		Expressed in the walls of the renal pelvis.
TS20: renal capsule	Absent
TS20: retina	Absent
TS20: urogenital ridge	Present	Regionally restricted		Expressed in the mesothelial lining.
TS20: mesonephros	Present	Regionally restricted		Expressed in the interstitium.
TS20: mesonephric tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS20: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS20: epidermis	Ambiguous			Relative lack of staining.
TS20: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.

Structure	Level	Pattern	Image	Note
TS23: Bowman's capsule of maturing renal corpuscle	Present
TS23: endocardial tissue	Present
TS23: glomerular basement membrane of maturing renal corpuscle	Present
TS23: glomerular mesangium of maturing glomerular tuft	Present
TS23: pericardium	Present
TS23: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: renal tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS23: renal interstitium	Present	Regionally restricted		Expressed in tubular interstium.
TS23: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS23: epidermis	Ambiguous			Relative lack of staining.
TS23: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.

Structure	Level	Pattern	Image	Note
TS24: Bowman's capsule of maturing renal corpuscle	Present
TS24: endocardial tissue	Present
TS24: pericardium	Present
TS24: tunica albuginea of testis	Present
TS24: vasculature of bladder	Present
TS24: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: maturing glomerular tuft	Present	Regionally restricted		Expressed in the glomerular basement membrane, the glomerular mesangium and the walls of glomerular capillaries.
TS24: renal tubule	Present	Regionally restricted		Expressed in the tubular basement membrane and tubular interstium.
TS24: bladder	Present	Regionally restricted		Expressed in the epitheial basement membrane and the subepithelial connective tissue.
TS24: retina	Absent
TS24: urothelium of bladder	Absent
TS24: detrusor muscle of bladder	Present	Regionally restricted		Expressed in the enveloping smooth muscle.
TS24: seminiferous cord	Present	Regionally restricted		Expressed in the basement membrane.
TS24: testis	Present	Regionally restricted		Expressed in the interstitium.
TS24: Meckel's cartilage	Present	Regionally restricted		Expressed in the peripheral in addition to diffuse matrix staining.
TS24: metatarsus	Present	Regionally restricted		Expressed in the ends of the bones.
TS24: adenohypophysis pars anterior	Present	Regionally restricted		Expressed in the blood vessels, not the parenchyma.
TS24: thyroid gland	Present	Regionally restricted		Expressed in the basement membranes and the vascular interstitium.
TS24: adrenal gland	Present	Regionally restricted		Expressed in the capsule and the interstitium.
TS24: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS24: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS24: epidermis	Ambiguous			Relative lack of staining.