Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1.1(CAG-tdTomato,-EGFP)Pjen
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Patricia Jensen
MGI ID:	MGI:5617961
Synonyms:	RC::RFLTG
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1.1(CAG-tdTomato,-EGFP)Pjen page

Germline Transmission:	Earliest citation of germline transmission: J:226320
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a rox-flanked His3-SV40 transcriptional STOP cassette, a frt-flanked His3-SV40 transcriptional STOP cassette, a loxP site, a cDNA sequence encoding tdTomato fluorescent protein, a His3-SV40 transcriptional STOP cassette, a bovine growth hormone polyA signal, a second loxP site, a cDNA sequence encoding enhanced green fluorescent protein (eGFP), a woodchuck hepatitis virus post-transcriptional regulatory element, a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. Transient transfection with a PhiC31-expressing plasmid removes the attB/attP-flanked PGK-frt-Neo-polyA cassette and replaces it with the recombined attB/attP site (attL). (J:226320)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:226320 Plummer NW, et al., Expanding the power of recombinase-based labeling to uncover cellular diversity. Development. 2015 Dec 15;142(24):4385-93
All:	3 reference(s)