Antibody:	anti-Fras1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Regionally restricted	1D	Authors report expression at the dermal epidermal interface, the subepithelial matrix of single-layered epidermis.

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present	Regionally restricted	1E	Expression at the subepithelial matrix of the two-layered epidermis.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	1F	Expression at the subepithelial matrix of the three-layered epidermis.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	2B	Expression strictly localized in the basement membrane.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	2D	Expression exclusively in the basement membrane.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Present	Regionally restricted	2F	Expression exclusively in the basement membrane.

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present	Regionally restricted	3B	Expression in the basement membrane of the protruding eyelid.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present	Regionally restricted	4D	Expression is strictly localized in basement membrane.

Structure	Level	Pattern	Image	Note
TS23: tooth	Present	Regionally restricted	4H	Expression in basement membrane but not in epithelial or mesenchymal cells.
TS23: tooth epithelium	Absent		4H
TS23: tooth mesenchyme	Absent		4H

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	5D	Expression in the basement membrane of respiratory epithelia.
TS22: visceral pleura	Present		5D	Expression in the mesothelial layer of visceral pleura.

Structure	Level	Pattern	Image	Note
TS22: metanephros epithelium	Strong	Regionally restricted	5H	Expression in the epithelial basement membranes.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present	Regionally restricted	5L	Espression in epithelial basement membranes.
TS22: gut mesentery	Present	Regionally restricted	5L	Expression in intestinal mesothelium.