Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO-Nog,-EGFP)53Hda
Name:	transgene insertion 53, K Sue O'Shea
MGI ID:	MGI:5609523
Synonyms:	pBi-Noggin-EGFP
Transgene:	Tg(tetO-Nog,-EGFP)53Hda  Location: unknown  Genetic Position: Chr16, Syntenic
Alliance:	Tg(tetO-Nog,-EGFP)53Hda page

Strain of Origin:

FVB/N-Tg(Nes-rtTA)306Rvs/J

Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Tg(tetO-Nog,-EGFP)53Hda involves 1 genes/genome features (Golgb1) View all   Tg(tetO-Nog,-EGFP)53Hda expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Nog (MGI:104327)   Mutation details: The transgene has a Pbi-1 bi-directional tetracycline-responsive promoter with an enhanced green fluorescent protein-coding region (eGFP) on one side, and a mouse noggin cDNA sequence (encompassing the complete coding region) on the other side. The promoter contains the Tet-responsive element (TRE) placed between two constitutively active, minimal human cytomegalovirus promoters. The eGFP is followed by a beta-globin polyA signal, and the noggin cDNA is followed by an SV40 polyA signal. Analysis at The Jackson Laboratory revealed two complex transgene integration sites (including one transgene-transgene fusion event) both within the Golgb1 locus on chromosome 16. This two duplications of Golgb1 genome region (totaling ~22.3 kb) are fused to transgene sequences (chr16:36,898,704-36,921,071 [mm10]) and chr16:36,899,753-36,899,756 [mm10]). The number of transgene copies at each site was not determined. The transgene insertion effect on Golgb1 expression has not been specifically determined. (J:216792)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:216792 Morell M, et al., Inducible expression of noggin selectively expands neural progenitors in the adult SVZ. Stem Cell Res. 2014 Nov 22;14(1):79-94
All:	1 reference(s)