Pcyt2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212402 Pavlovic Z, et al., A novel murine CTP:phosphoethanolamine cytidylyltransferase splice variant is a post-translational repressor and an indicator that both cytidylyltransferase domains are required for activity. Gene. 2014 Jun 10;543(1):58-68
Assay type:	RT-PCR
MGI Accession ID:	MGI:5607631
Gene symbol:	Pcyt2
Gene name:	phosphate cytidylyltransferase 2, ethanolamine

Probe:	Pcyt2-pA, Pcyt2-pB
Assay notes:	The primer set used amplifies three transcript isoforms. They are all ubiquitously expressed but at different levels which are quantified elsewhere the in the figure.

Results

Image: 2A

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	408 bp (a)	224 bp (b)	170 bp (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
L	Mol. Wt. Marker Lane
1	P adult	TS28: heart	Present (d)	Present	Present	Not Specified	Not Specified		Not Specified
2	P adult	TS28: brain	Present (d)	Present	Present	Not Specified	Not Specified		Not Specified
3	P adult	TS28: spleen	Present	Present	Present	Not Specified	Not Specified		Not Specified
4	P adult	TS28: lung	Present	Present	Present	Not Specified	Not Specified		Not Specified
5	P adult	TS28: liver	Present	Present (e)	Present	Not Specified	Not Specified		Not Specified
6	P adult	TS28: skeletal muscle	Present	Present	Present	Not Specified	Not Specified		Not Specified
7	P adult	TS28: metanephros	Present (e)	Present (e)	Present	Not Specified	Not Specified		Not Specified
8	P adult	TS28: testis	Present	Present	Present	Not Specified	Not Specified		Male
L	Mol. Wt. Marker Lane
7E	E7.0 (f)	TS10: embryo	Present	Present	Present	Not Specified	Not Specified		Not Specified
11E	E11.0 (f)	TS18: embryo	Present	Present	Present	Not Specified	Not Specified		Not Specified
15E	E15.0 (f)	TS23: embryo	Present	Present	Present	Not Specified	Not Specified		Not Specified
17E	E17.0 (f)	TS25: embryo	Present	Present	Present	Not Specified	Not Specified		Not Specified

Notes:
(a) This band represents the gamma isoform.
(b) This band represents the alpha isoform; it is the predominantly expressed isoform.
(c) This band represents the beta isoform.
(d) This is one of the tissues with the lowest level of expression of this isoform.
(e) This is one of the tissues with the highest level of expression of this isoform.
(f) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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