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Gene Expression Data
Assay Details
Assay
Reference: J:211539 Chen Y, et al., Compensatory regulation of the Snai1 and Snai2 genes during chondrogenesis. J Bone Miner Res. 2013 Jun;28(6):1412-21
Assay type: RT-PCR
MGI Accession ID: MGI:5603139
Gene symbol: Trp53
Gene name: transformation related protein 53
Probe: Trp53-pE, Trp53-pF
Probe preparation: labelled with SYBR green
Assay notes: Real Time PCR was used to obtain these results. Three experimental replicates and four biological replicates were used. Gene expression levels were normalized to the beta-actin mRNA level.
Results Image: S2_Trp53
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
LC E16.5 TS24: femur Present 2 µg; total RNA involves: 129S1/Sv * C57BL/6J Snai1tm2Grid/Snai1tm2GridNot Specified littermate controls
Snai1 MT E16.5 TS24: femur Present 2 µg; total RNA involves: 129S1/Sv * C57BL/6J Snai1tm1Grid/Snai1tm1Grid, Tg(Prrx1-cre)1Cjt/0Not Specified Conditional mutant.
Snai2 MT E16.5 TS24: femur Present 2 µg; total RNA involves: 129S1/Sv * C57BL/6J Snai1tm2Grid/Snai1tm2Grid, Snai2tm1Grid/Snai2tm1GridNot Specified
Snai1/Snai2 MT E16.5 TS24: femur Present (a) 2 µg; total RNA involves: 129S1/Sv * C57BL/6J Snai1tm1Grid/Snai1tm1Grid, Snai2tm1Grid/Snai2tm1Grid, Tg(Prrx1-cre)1Cjt/0Not Specified Conditional mutant.
Notes:
(a) Expression levels are significantly upregulated in the double mutant relative to controls and single mutants.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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