Gene Expression Data
Assay Details
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Assay
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Reference:
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J:212101
Kamiya K, et al., An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cells. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9307-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5602953
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Gene symbol:
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Strc
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Gene name:
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stereocilin
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Antibody:
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anti-stereocilin
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Assay notes:
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The emission color of the secondary used is green.
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Results
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Specimen
2D wild-type:
postnatal
(more  )
Specimen
2D wild-type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Specimen
2D Nherf1-/-:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nherf1tm1Geo/Nherf1tm1Geo
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
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2D lower
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In the mutant the sterocilia are anchored in the tectorial membrane properly. However, the imprint of the hair cells in the tectorial membrane is severely misshapen, indicating the hair bundles have abnormal shapes.
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Specimen
S5E wild-type:
postnatal
(more )
Specimen
S5E wild-type:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Specimen
S5E Nherf1-/-:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nherf1tm1Geo/Nherf1tm1Geo
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Specimen
cochlear base:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nherf1tm1Geo/Nherf1tm1Geo
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
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S5E right upper
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In the mutant the sterocilia are anchored in the tectorial membrane properly. However, for 90% of the hair cells in the cochlear base the imprint is severely misshapen, indicating the hair bundles have abnormal shapes.
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Specimen
cochlear apex:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Nherf1tm1Geo/Nherf1tm1Geo
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
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S5E right lower
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In the mutant the sterocilia are anchored in the tectorial membrane properly. In addition, in the cochlear apex, unlike the base, most hair bundles are properly shaped. Only 15% show any abnormalities, and those abnormalities are minor.
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Analysis Tools