Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Sec24dtm1d(EUCOMM)Hmgu
Name:	SEC24 homolog D, COPII coat complex component; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
MGI ID:	MGI:5582287
Gene:	Sec24d  Location: Chr3:123061104-123159290 bp, + strand  Genetic Position: Chr3, 53.82 cM, cytoband G3
Alliance:	Sec24dtm1d(EUCOMM)Hmgu page
IMPC:	Sec24d gene page

Mutant Cell Line:	HEPD0753_7_H04
Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 123086679 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123088075. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Flp recombinase, leaving exons 5 and 6 flanked by loxP sites. Then animals were crossed with a germline EIIa-Cre strain to delete exons 5 and 6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sec24d Mutation:	59 strains or lines available

Original:	J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:	2 reference(s)