Slit3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:166534 Medioni C, et al., Expression of Slit and Robo genes in the developing mouse heart. Dev Dyn. 2010 Dec;239(12):3303-11
Assay type:	RNA in situ
MGI Accession ID:	MGI:5581448
Gene symbol:	Slit3
Gene name:	slit guidance ligand 3

Probe:	mSlit3 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1I ©

Specimen 1I: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: somite	Present		1I
TS13: heart	Present		1I	Expression is in the linear heart tube.
TS13: embryo	Present	Regionally restricted	1I	Expression is in the ventral midline.

1I' ©

Specimen 1I': embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: heart	Present		1I'	Expression is in the cardiac crescent.

1J ©

Specimen 1J: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: branchial arch ectoderm	Present		1J
TS14: somite	Present		1J
TS14: heart	Present	Regionally restricted	1J	Expression is restricted to the outer curvature.

1J' ©

Specimen 1J': embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: branchial arch ectoderm	Present		1J'

1K ©

Specimen 1K: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		1K
TS15: heart atrium	Present	Regionally restricted	1K	Expression is restricted to the outer curvature.
TS15: primitive ventricle	Present	Regionally restricted	1K	Expression is restricted to the outer curvature.

1L ©

Specimen 1L: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		1L
TS20: left atrium cardiac muscle	Present		1L
TS20: pulmonary artery	Present		1L
TS20: right atrium cardiac muscle	Present		1L
TS20: arterial system	Present	Regionally restricted	1L	Expression is at the base of the great arteries.

1L' ©

Specimen 1L': embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		1L'
TS20: left atrium cardiac muscle	Present		1L'
TS20: pulmonary artery	Present		1L'
TS20: right atrium cardiac muscle	Present		1L'
TS20: arterial system	Present	Regionally restricted	1L'	Expression is at the base of the great arteries.

3A ©

Specimen 3A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Strong	Regionally restricted	3A	Expression is in the ventral side.

3B ©

Specimen 3B: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Strong	Regionally restricted	3B	Expression is in the ventral side.

3D ©

Specimen 3D: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	3D	Expression is in the ventral side.

3D' ©

Specimen 3D': embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	3D'	Expression is in the ventral side.
TS13: neural tube floor plate	Present		3D'

3E ©

Specimen 3E: embryonic day 8.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present		3E	Expression in this mutant is the same as in wild-type embryos.

3F ©

Specimen 3F: embryonic day 8.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Uniform	3F

4A ©

Specimen 4A: embryonic day 9.0; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Pattern	Image	Note
TS14: heart	Present		4A
TS14: atrioventricular canal	Absent		4A

4B ©

Specimen 4B: embryonic day 9.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Pattern	Image	Note
TS15: heart	Present		4B
TS15: atrioventricular canal	Absent		4B

4C ©

Specimen 4C: embryonic day 9.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Image	Note
TS15: heart atrium	Present	4C
TS15: primitive ventricle	Present	4C	Expression is weaker in the trabeculae of the left ventricle.
TS15: atrioventricular canal	Absent	4C

4D ©

Specimen 4D: embryonic day 10.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Image
TS17: common atrial chamber left part	Strong	4D
TS17: dermomyotome	Present	4D
TS17: limb	Present	4D

4E ©

Specimen 4E: embryonic day 10.5; Nkx2-5^tm4Rph/Nkx2-5^tm4Rph (more

)

Structure	Level	Image
TS17: common atrial chamber left part	Strong	4E
TS17: common atrial chamber right part	Strong	4E
TS17: outflow tract	Strong	4E

5A ©

Specimen 5A: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: heart	Present		5A

5A' ©

Specimen 5A': embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: heart	Present		5A'

5B ©

Specimen 5B: embryonic day 9.0; Tbx20^tm1.1Rph/Tbx20^tm1.1Rph (more

)

Structure	Level	Pattern	Image	Note
TS14: heart	Absent		5B

5B' ©

Specimen 5B': embryonic day 9.0; Tbx20^tm1.1Rph/Tbx20^tm1.1Rph (more

)

Structure	Level	Pattern	Image	Note
TS14: heart	Absent		5B'

S4A ©

Specimen S4A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Absent		S4A

S4B ©

Specimen S4B: embryonic day 10.5; Tbx2^tm1Pa/Tbx2^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present		S4B

S4C ©

Specimen S4C: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: heart	Present	Regionally restricted	S4C
TS15: atrioventricular canal	Absent		S4C

S4D ©

Specimen S4D: embryonic day 9.5; Tbx3^tm1Pa/Tbx3^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS15: heart	Present		S4D	Expression in this mutant is the same as in wild-type embryos.
TS15: atrioventricular canal	Absent		S4D	Absence of expression in this mutant is the same as in wild-type embryos.

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