Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:124197
Kiyozumi D, et al., Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1. Matrix Biol. 2007 Jul;26(6):456-462
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5578525
|
|
Gene symbol:
|
Fras1
|
|
Gene name:
|
Fraser extracellular matrix complex subunit 1
|
|
Antibody:
|
Anti-Fras1
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Assay notes:
|
Cryosections were fixed in absolute ethanol.
|
|
|
Results
|
Specimen
2B:
postnatal week 9
(more  )
Note: Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
Specimen
2B:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 9
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
|
Specimen
2C:
embryonic day 14.5
(more )
Note: Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
|
Specimen
2D:
postnatal adult
(more )
Note: Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
Specimen
2D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
|
Specimen
2E:
embryonic day 14.5
(more )
Note: Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
Specimen
2E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metanephros |
Present |
Regionally restricted |
2E Fras1
|
Expression is at the renal epithelial basement membrane.
|
Specimen
3A:
embryonic day 17.5
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Regionally restricted |
3A Fras1
|
Expression is at the interfollicular epidermal basement membrane.
|
| TS25: hair follicle |
Strong |
Regionally restricted |
3A Fras1
|
Expression is at the basement membrane engulfing dermal papilla.
|
Specimen
3B:
postnatal week 4
(more )
Note: Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
Specimen
3B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Fras1; red - Lamc1 (assay MGI:5578519).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: coat hair follicle |
Present |
Regionally restricted |
3B Fras1
|
Expression is at the basement membrane engulfing dermal papilla.
|
| TS28: epidermis |
Trace |
Regionally restricted |
3B Fras1
|
Expression is at the interfollicular epidermal basement membrane.
|
| TS28: hair outer root sheath |
Trace |
Regionally restricted |
3B Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Qbrick/Frem1+/-:
postnatal newborn; Frem1tm1Ksek/Frem1 +
(more )
Specimen
4A Qbrick/Frem1+/-:
(close )
|
Genetic Background:
|
involves: 129
|
|
Mutant Allele(s):
|
Frem1tm1Ksek/Frem1+
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Qbrick/Frem1+/- Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Qbrick/Frem1-/-:
(close )
|
Genetic Background:
|
involves: 129
|
|
Mutant Allele(s):
|
Frem1tm1Ksek/Frem1tm1Ksek
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Weak |
Regionally restricted |
4A Qbrick/Frem1-/- Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Frem2my/+:
postnatal newborn; Frem2my/Frem2 +
(more )
Specimen
4A Frem2my/+:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Frem2my/Frem2+
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Frem2my/+ Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Frem2my/my:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Frem2my/Frem2my
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Weak |
Regionally restricted |
4A Frem2my/my Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Grip1eb/+:
postnatal newborn; Grip1eb/Grip1 +
(more )
Specimen
4A Grip1eb/+:
(close )
|
Genetic Background:
|
involves: hairless stock
|
|
Mutant Allele(s):
|
Grip1eb/Grip1+
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Grip1eb/+ Fras1
|
Expression is at the basement membrane.
|
Specimen
4A Grip1eb/eb:
(close )
|
Genetic Background:
|
involves: hairless stock
|
|
Mutant Allele(s):
|
Grip1eb/Grip1eb
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Weak |
Regionally restricted |
4A Grip1eb/eb Fras1
|
Expression is at the basement membrane.
|
|
Analysis Tools