Probe:	Satb2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors used the same probes as in J:85953. Two probes were described, but the one used in this assay was not detailed. Satb2 cDNA3 MGI:5576590 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2A	Expression is in the distal regions.
TS17: medial-nasal process mesenchyme	Present	Regionally restricted	2A	Expression is in the distal regions.
TS17: 1st branchial arch maxillary component mesenchyme	Present	Regionally restricted	2A	Expression is at the oral side.

Structure	Level	Pattern	Image	Note
TS19: medial-nasal process mesenchyme	Present		2B
TS19: mandibular process mesenchyme	Present	Regionally restricted	2B	Expression is at the oral side.
TS19: maxillary process mesenchyme	Present	Regionally restricted	2B	Expression is more medially.

Structure	Level	Pattern	Image	Note
TS19: maxillary process mesenchyme	Present	Regionally restricted	2C	Expression is at the oral side.
TS19: maxillary process	Present	Regionally restricted	2C	Expression is in the incisor tooth germ.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Strong	Regionally restricted	2D	Expression is along the entire anterior-posterior axis.
TS21: upper lip	Strong		2D
TS21: mandible	Weak		2D

Structure	Level	Pattern	Image	Note
TS22: palatal shelf	Present	Regionally restricted	2E	Expression is in the most posterior region where the shelves have not yet adhered.
TS22: upper lip	Present		2E

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Absent		2F	Expression is absent after the palate has fused.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		2G
TS17: hindlimb bud apical ectodermal ridge	Present		2G

Structure	Level	Pattern	Image	Note
TS20: tongue	Present		2H

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Strong		3A
TS20: mandible primordium	Strong		3A
TS20: palatal shelf mesenchyme	Strong		3A
TS20: tongue	Strong		3A

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Strong		3B
TS21: mandible	Strong		3B
TS21: palatal shelf mesenchyme	Strong		3B , 3C
TS21: tongue mesenchyme	Strong		3B , 3C
TS21: palatal shelf epithelium	Absent		3C , 3B
TS21: tongue epithelium	Absent		3C , 3B

Structure	Level	Pattern	Image	Note
TS22: tongue	Weak		3D
TS22: palatal shelf mesenchyme	Present	Regionally restricted	3D	Expression is in the zones of ossification of the future palatine bone and is weak in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: upper lip	Present		S1A
TS21: mandible	Present		S1A

Structure	Level	Pattern	Image	Note
TS22: upper lip	Present		S1B
TS22: mandible	Weak		S1B

Structure	Level	Pattern	Image	Note
TS20: palatal shelf	Present		S1C
TS20: oral region	Present	Regionally restricted	S1C	Expression is in the upper lip.