Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:84397
Yang J, et al., Nestin expression during mouse eye and lens development. Mech Dev. 2000 Jun;94(1-2):287-91
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5575906
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Gene symbol:
|
Nes
|
Gene name:
|
nestin
|
Probe:
|
Nes cDNA6
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
2A:
embryonic day 9.0
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: eye |
Present |
Regionally restricted |
2A
|
Expression is in the luminal side of the optic stalk and in the surface ectoderm overlying the optic vesicle.
|
Specimen
2B:
embryonic day 10.0
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2D:
embryonic day 14.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens fiber |
Present |
Regionally restricted |
2D
|
Expression is in primary lens fibers.
|
Specimen
2E:
embryonic day 17.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2F:
postnatal day 1
(more )
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens fiber |
Present |
Regionally restricted |
3A
|
Expression is in primary lens fiber cells.
|
Specimen
3B:
embryonic day 14.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens fiber |
Present |
Regionally restricted |
3B
|
Expression is in primary lens fiber cells.
|
Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens fiber |
Present |
Regionally restricted |
3C
|
Expression is in primary lens fiber cells.
|
Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: eye |
Absent |
|
|
|
|