Antibody:	anti-nestin
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Strong	Regionally restricted	1A	Expression is in the neuroepithelium.
TS15: optic vesicle	Present	Graded	1A	There is a gradual posterior-to-anterior decrease in expression in the outer layer of the optic vesicle. Absent in the inner layer.
TS15: lens placode	Absent		1A

Structure	Level	Pattern	Image	Note
TS17: lens pit	Strong	Regionally restricted	1B	Expression is in the posterior lens epithelium.
TS17: optic cup	Weak	Regionally restricted	1B	Expression is in the inner layer of the neural retina.
TS17: optic stalk	Weak	Regionally restricted	1B	Expression is in the endfeet of the curved portion of the optic stalk.

Structure	Level	Pattern	Image	Note
TS20: cornea epithelium	Strong		1C
TS20: lens vesicle epithelium	Strong		1C
TS20: neural retinal epithelium	Strong	Regionally restricted	1C	Expression is in the inner layer.
TS20: eye	Present	Regionally restricted	1C	Expression is at the border between optic stalk and the surrounding tissues including future extrinsic occular muscle.
TS20: optic stalk	Present		1C

Structure	Level	Pattern	Image	Note
TS22: lens anterior epithelium	Present	Regionally restricted	1D	Expression is at the endfeet.
TS22: optic disc	Weak		1D
TS22: optic nerve	Weak		1D

Structure	Level	Pattern	Image	Note
TS25: ciliary body	Strong		1E
TS25: lens epithelium	Strong		1E
TS25: optic disc	Strong		1E
TS25: optic nerve	Strong		1E
TS25: posterior lens fiber	Strong		1E
TS25: neural retina	Strong	Regionally restricted	1E	Expression is in the outer layer.

Structure	Level	Pattern	Image	Note
TS27: lens anterior epithelium	Present		1F
TS27: optic disc	Present		1F

Structure	Level	Pattern	Image	Note
TS28: optic nerve	Strong		1G

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present	Regionally restricted	3D	Expression is at the apical endfeet of lens epithelial cells.