Probe:	Cxcl14 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Two probes were described, but the one used in this assay was not detailed. Probe Cxcl14 cDNA3 MGI:5574456 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		1A
TS15: telencephalon	Present		1A
TS15: embryo ectoderm	Present	Regionally restricted	1A	Expression is ventral.
TS15: future midbrain	Present	Regionally restricted	1A	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1B
TS17: glossopharyngeal ganglion	Present		1B
TS17: otocyst	Present		1B
TS17: vagus ganglion	Present		1B
TS17: trunk	Present	Regionally restricted	1B	Expression is between the somites.
TS17: limb mesenchyme	Present	Regionally restricted	1B	Expression is in a central region.

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Strong		1C
TS19: limb mesenchyme	Strong		1C
TS19: trunk	Present	Regionally restricted	1C	Expression is between the somites.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present		1D

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4A	Expression is in the anterior-posterior axis with a weaker signal in the posterio-distal most mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	4B	The dorsal expression domain has branched distally. Stronger expression is in the posterio-distal domain.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		4C

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present	Regionally restricted	4D	Expression is in the connective tissue surrounding the chondrogenic regions.
TS21: forelimb digit	Present	Regionally restricted	4D	Expression is in the interphalangeal joints.
TS21: forelimb pre-cartilage condensation	Absent		4D

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5C	Expression is in two populations, one underlying the dorsal ectoderm and a second underlying the ventral ectoderm.
TS17: forelimb bud ectoderm	Present	Graded	5C	Expression is strong proximally and fades distally.
TS17: forelimb bud apical ectodermal ridge	Absent		5C

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		5E

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present		6A	Expression is in the connective tissue peripheral to the ventral and posterior muscle masses.
TS21: forearm epithelium	Present		6A

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	6F	Expression is in all ventral connective tissue extending into the periphery of tendinous bundles.
TS21: hand mesenchyme	Present	Regionally restricted	6F	Expression is in mesenchyme associated with large vessels.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present	Regionally restricted	6K	Expression is associated with the periphery of the tendons with stronger expression in the ventral tendons.

Structure	Level	Pattern	Image	Note
TS21: forearm	Present		6P	Expression is associated with the periphery of the ventral tendon.