Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Polr2aTn(pb-CAG-GCaMP5g,-tdTomato)Tvrd
Name:	polymerase (RNA) II (DNA directed) polypeptide A; transposon insertion 1, Petr Tvrdik
MGI ID:	MGI:5560335
Synonyms:	PC::G5-tdT
Gene:	Polr2a  Location: Chr11:69624823-69649459 bp, - strand  Genetic Position: Chr11, 42.86 cM, cytoband B1-C
Alliance:	Polr2aTn(pb-CAG-GCaMP5g,-tdTomato)Tvrd page

Germline Transmission:	Earliest citation of germline transmission: J:212315
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Transposon induced (Conditional ready, No functional change, Reporter) Mutation:    Transposon insertion   Mutation details: The transposon contains a floxed STOP cassette followed by a NEO selection cassette in opposite orientation, the genetically encoded calcium indicator GCaMP5g, a FRT-flanked IRES-tdTomato, and woodchuck hepatitis posttranscriptional regulatory element (WPRE), under the control of the CAG promoter. This construct was flanked by piggyBAC transposase inverted terminal repeats and the entire reporter cassette was inserted approximately 1 kb 3' of the last exon of the Polr2a gene. (J:101977, J:212315)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Polr2a Mutation:	92 strains or lines available

Original:	J:212315 Gee JM, et al., Imaging activity in neurons and glia with a Polr2a-based and cre-dependent GCaMP5G-IRES-tdTomato reporter mouse. Neuron. 2014 Sep 3;83(5):1058-72
All:	31 reference(s)