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Gene Expression Data
Assay Details
Assay
Reference: J:202055 Siyanov V, et al., NHE1 is the sodium-hydrogen exchanger active in acute intracellular pH regulation in preimplantation mouse embryos. Biol Reprod. 2013 Jun;88(6):157
Assay type: RT-PCR
MGI Accession ID: MGI:5553331
Gene symbol: H2az1
Gene name: H2A.Z variant histone 1
Probe: H2afz-pA, Hsafz-pB
Visualized with: Ethidium bromide
Assay notes: These reactions were repeated on at least four separate oocyte or embryo collections. Each reaction contained an amount of cDNA template equal to 1.5 oocytes or embryos.
Results Image: 1A H2afz
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 Mol. Wt. Marker Lane
[+] Control
[-] Control
GV P (a) TS28: primary oocyte Present Not Specified CF-1 Female germinal vesicle oocytes
E P (a) TS28: secondary oocyte Present Not Specified CF-1 Female metaphase II eggs
1C E0.5 (a) TS1: 1-cell stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified one-cell embryos
2C E1.0 (a) TS2: 2-cell stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified two-cell embryos
4C E2.0 (a) TS3: 4-cell stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified four-cell embryos
8C E2.5 (a) TS3: 8-cell stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified eight-cell embryos
M E3.0 (a) TS3: morula-stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified morulae
B E3.5 (a) TS4: blastocyst-stage conceptus Present Not Specified involves: C57BL/6 * CF-1 * DBA/2 Not Specified blastocysts
Lane 12 Mol. Wt. Marker Lane
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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