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Gene Expression Data
Assay Details
Assay
Reference: J:108819 Miyoshi T, et al., Complementary expression pattern of Zfhx1 genes Sip1 and deltaEF1 in the mouse embryo and their genetic interaction revealed by compound mutants. Dev Dyn. 2006 Apr 5;235(7):1941-1952
Assay type: Northern blot
MGI Accession ID: MGI:5550494
Gene symbol: Zeb1
Gene name: zinc finger E-box binding homeobox 1
Probe: delta-EF1 cDNA1
Probe preparation: Double stranded
Assay notes: Hybridization with the probe produced a major band of 5.8 kb (solid arrows), and longer band (open arrow), derived from alternative transcriptional termination sites.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 5.8 kb Amount Genetic BackgroundMutant Allele(s)Sex Note
wild type E8.5 TS13: embryo Present 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * ICR Not Specified 6 somite
Sip1+/- E8.5 TS13: embryo Present (a) 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * ICR Zeb2tm1.2Yhi/Zeb2+Not Specified 6 somite
Sip1-/- E8.5 TS13: embryo Present (b) 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * ICR Zeb2tm1.2Yhi/Zeb2tm1.2YhiNot Specified 6 somite
deltaEF1-/- E8.5 TS13: embryo Absent 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * ICR Zeb1tm2Yhi/Zeb1tm2YhiNot Specified 6 somite
Notes:
(a) 1.19-fold increase, compared to wild-type embryos.
(b) 1.35-fold increase, compared to wild-type embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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