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Gene Expression Data
Assay Details
Assay
Reference: J:119468 Kedees MH, et al., Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. Dev Dyn. 2007 Mar 15;236(4):1126-1133
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5550100
Gene symbol: Ins1
Gene name: insulin I
Antibody: anti-insulin
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 1E,G: embryonic day 11.0 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS18: pancreas primordium Present Single cells 1G , 1E Few cells were immunopositive. An occasional immunopositive cell co-expressed Nestin.
Specimen 2A: embryonic day 15.0; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS23: pancreas Present Regionally restricted 2A Co-expression with Nestin was noted in a large number of cells. 30% of insulin-positive cells expressed Nestin.
Specimen 2C: embryonic day 15.0; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Structure Level Pattern Image Note
TS23: pancreas Present 2C
Specimen 2D: embryonic day 15.0 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS23: pancreas Present 2D
Specimen 3A: postnatal day 1; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS27: pancreas Present 3A The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the PC2 mutant P1 islets.
Specimen not shown: postnatal day 1; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Note: Also stained for Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS27: pancreas Present The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the Gcgr mutant P1 islets.
Specimen 4A: postnatal month 3; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4A Some cells co-expressed Nestin.
Specimen 4F: postnatal month 3 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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