Ins1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:119468 Kedees MH, et al., Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. Dev Dyn. 2007 Mar 15;236(4):1126-1133
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5550100
Gene symbol:	Ins1
Gene name:	insulin I

Antibody:	anti-insulin
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Results

1E ©

1G ©

Specimen 1E,G: embryonic day 11.0 (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS18: pancreas primordium	Present	Single cells	1G , 1E	Few cells were immunopositive. An occasional immunopositive cell co-expressed Nestin.

2A ©

Specimen 2A: embryonic day 15.0; Gcgr^tm1Mjch/Gcgr^tm1Mjch (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	2A	Co-expression with Nestin was noted in a large number of cells. 30% of insulin-positive cells expressed Nestin.

2C ©

Specimen 2C: embryonic day 15.0; Gcgr^tm1Mjch/Gcgr^tm1Mjch (more

)

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		2C

2D ©

Specimen 2D: embryonic day 15.0 (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present		2D

3A ©

Specimen 3A: postnatal day 1; Pcsk2^tm1Dfs/Pcsk2^tm1Dfs (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		3A	The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the PC2 mutant P1 islets.

Specimen not shown: postnatal day 1; Gcgr^tm1Mjch/Gcgr^tm1Mjch (more

)
Note: Also stained for Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present			The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the Gcgr mutant P1 islets.

4A ©

Specimen 4A: postnatal month 3; Pcsk2^tm1Dfs/Pcsk2^tm1Dfs (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present		4A	Some cells co-expressed Nestin.

4F ©

Specimen 4F: postnatal month 3 (more

)
Note: Double labeled: red - Ins1 and Ins2; green - Nes (assay MGI:5550086).

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present		4F

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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