Summary | Mutation origin | Mutation description | Expression | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Pkmtm1.1Mgvh
Name:	pyruvate kinase, muscle; targeted mutation 1.1, Matthew G Vander Heiden
MGI ID:	MGI:5547750
Synonyms:	Pkm2fl
Gene:	Pkm  Location: Chr9:59563859-59586655 bp, + strand  Genetic Position: Chr9, 32.03 cM
Alliance:	Pkmtm1.1Mgvh page

Germline Transmission:	Earliest citation of germline transmission: J:205213
Parent Cell Line:	TC1/TC-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Conditional ready, Modified isoform(s), No functional change) Mutation:    Insertion   Mutation details: A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10 which is alternatively spliced only in the Pkm2 isoform. Mutant mice were bred with mice expressing FlpO recombinase (Gt(ROSA)26Sor) to remove the neomycin selection cassette. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is eliminated, while pyruvate kinase M1 isoform expression is unchanged. (J:205213)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

15 assay results

List all tumor models in MMHCdb carrying Pkmtm1.1Mgvh

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Pkm Mutation:	75 strains or lines available

Original:	J:205213 Israelsen WJ, et al., PKM2 isoform-specific deletion reveals a differential requirement for pyruvate kinase in tumor cells. Cell. 2013 Oct 10;155(2):397-409
All:	41 reference(s)