Probe:	Zic4 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Weak	Regionally restricted	1A	Expression is in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong		1B	Expression is dorsal.
TS15: telencephalon	Strong		1B	Expression is dorsal.
TS15: dermomyotome	Present	Regionally restricted	1B	Expression is in the dorsomedial lip.
TS15: sclerotome	Present		1B	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Weak	Regionally restricted	1C	Expression is in the dorsal midline.
TS14: future hindbrain	Weak	Regionally restricted	1C	Expression is in the dorsal region of the low hindbrain.
TS14: neural tube	Weak	Regionally restricted	1C	Expression is dorsal in the upper spinal neural tube.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1D	Expression is dorsal in the upper spinal neural tube.
TS14: neural tube roof plate	Absent		1D

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1E	Expression is dorsal.
TS15: neural tube roof plate	Absent		1E

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1F	In this section, which is posterior to the forelimb bud, expression is absent. In more anterior sections expression is dorsal.
TS15: dermomyotome	Present	Regionally restricted	1F	Expression is in the dorsomedial lip.
TS15: sclerotome	Present	Regionally restricted	1F	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong	Regionally restricted	2A	Expression is at the dorsal midline.
TS17: neural tube	Strong	Regionally restricted	2A	Expression is dorsal and is absent in the extreme caudal region.
TS17: somite	Strong		2A
TS17: dorsal root ganglion	Absent		2A

Structure	Level	Pattern	Image	Note
TS17: brain	Strong	Regionally restricted	2B	Expression is dorsal.
TS17: neural tube	Strong	Regionally restricted	2B	Expression is dorsal.
TS17: telencephalon	Present	Regionally restricted	2B	Expression is in the septum and expression is at the dorsal part of the telencephalon/diencephalon boundary (prethalamus).
TS17: diencephalon lamina terminalis	Present		2B
TS17: diencephalon	Present	Regionally restricted	2B	Expression is at the dorsal part of the telencephalon/diencephalon boundary (prethalamus).

Structure	Level	Pattern	Image	Note
TS17: brain	Strong	Regionally restricted	2C	Expression is dorsal.
TS17: neural tube	Strong	Regionally restricted	2C	Expression is dorsal.
TS17: sclerotome	Present	Regionally restricted	2C	Expression is dorsal.
TS17: dermomyotome	Present	Regionally restricted	2C	Expression is in the dorsomedial dermomyotome.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2D	Expression is at telencephalon/diencephalon boundary.
TS17: telencephalon	Present	Regionally restricted	2D	Expression is at telencephalon/diencephalon boundary.

Structure	Level	Pattern	Image	Note
TS17: 3rd ventricle	Strong	Regionally restricted	2E	Expression is in the choroid plexus.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	2F	Expression is in the septum.
TS17: diencephalon lamina terminalis	Present		2F

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	2G	Expression is dorsal.
TS17: hindbrain roof plate	Present		2G
TS17: midbrain roof plate	Present		2G

Structure	Level	Pattern	Image	Note
TS17: neural tube roof plate	Present	Regionally restricted	2H	This section at the level of the heart shows expression is absent but it is present at the boundary of the hindbrain/spinal cord.
TS17: neural tube	Present	Regionally restricted	2H	Expression is dorsal and is absent in the caudal portion.
TS17: somite	Present		2H
TS17: dorsal root ganglion	Absent		2H

Structure	Level	Pattern	Image	Note
TS17: neural tube roof plate	Present	Regionally restricted	2I	This is a posterior section that shows expression is absent but it is present at the boundary of the hindbrain/spinal cord.
TS17: neural tube	Present	Regionally restricted	2I	Expression is dorsal.
TS17: somite	Strong		2I
TS17: dorsal root ganglion	Absent		2I

Structure	Level	Pattern	Image	Note
TS17: somite	Strong		2J
TS17: dorsal root ganglion	Absent		2J

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3B	Expression is in the septum.
TS20: choroid plexus	Present		3B

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3C	Expression is in the septum.
TS20: forebrain	Present	Regionally restricted	3C	Expression is in the medial edge of the hippocampal primordium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3D	Expression is in the septum.
TS20: forebrain	Present	Regionally restricted	3D	Expression is in the medial edge of the hippocampal primordium.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		3E
TS27: subcommissural organ	Present	Regionally restricted	3E	Expression is at the anterior end.
TS27: superior colliculus	Present	Regionally restricted	3E	Expression is at the anterior end.
TS27: thalamus	Present	Regionally restricted	3E	Expression is at the periventricular thalamus.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		3F

Structure	Level	Pattern	Image	Note
TS27: subcommissural organ	Present		3G
TS27: thalamus	Present	Regionally restricted	3G	Expression is at the periventricular thalamus.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	4D	Expression is along the dorsal midline.
TS15: neural tube	Present	Regionally restricted	4D	Expression is along the dorsal midline and is absent in the caudal spinal region.
TS15: somite	Present		4D

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4H	Expression is in the dorsal quarter of the neural tube.
TS15: sclerotome	Present		4H
TS15: neural tube roof plate	Absent		4H	The authors state expression is absent, but in Figure.

Structure	Level	Pattern	Image	Note
TS15: sclerotome	Present	Regionally restricted	4P , 4L	Expression is in the dorsal most part of the sclerotome.
TS15: dermomyotome	Present	Regionally restricted	4L , 4P	Expression is in the dorsomedial portion.
TS15: neural tube	Present	Regionally restricted	4L , 4P	In this section (mid trunk level) expression is absent in the neural tube. Expression is dorsal in anterior regions.