Probe:	Foxf2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		1a
TS13: lateral plate mesoderm	Present		1a
TS13: primitive streak	Present	Regionally restricted	1a	Expressed in the posterior primitive streak.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		1b
TS13: lateral plate mesoderm	Present		1b
TS13: primitive streak	Present	Regionally restricted	1b	Expressed in the posterior primitive streak.

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present	Regionally restricted	1c	Expressed in the splanchnic mesoderm of the hindgut but not around the foregut.

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present	Regionally restricted	1d	Expressed in the splanchnic mesoderm of the hindgut.
TS13: somatopleure	Absent		1d

Structure	Level	Pattern	Image	Note
TS15: allantois	Trace		2a right	Very weak expression.
TS15: yolk sac	Trace		2a right	Very weak expression.
TS15: trunk mesenchyme	Ambiguous		2a right	Hardly detetable in the lateral plate mesoderm.
TS15: head mesenchyme	Strong	Regionally restricted	2a right	Expressed in the mesenchyme surrounding Rathke's pouch and next to the oropharynx and stomodeum.
TS15: head mesenchyme derived from neural crest	Strong	Regionally restricted	2a right	Expressed in the mesenchyme surrounding Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2b	Expressed in mesenchyme between Rathke's pouch and the optic vesicles that evaginate from diencephalon.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme	Present		2c

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	2d	Expressed in splanchnic mesoderm around the foregut.
TS15: foregut mesenchyme	Present		2d

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	2e	Expressed in splanchnic mesoderm of hindgut.
TS15: hindgut diverticulum mesenchyme	Present		2e

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	2f	Higher expression in splanchnic mesoderm of hindgut than in mid- or foregut.
TS15: foregut mesenchyme	Present		2f
TS15: hindgut diverticulum mesenchyme	Present		2f
TS15: midgut mesenchyme	Present		2f

Structure	Level	Pattern	Image	Note
TS15: hindgut diverticulum mesenchyme	Present		2g

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	3a	Expressed in the caudal part.
TS19: forelimb bud	Present	Spotted	3a	Expressed as central dots.
TS19: hindlimb bud	Present	Spotted	3a	Expressed as central dots.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	3b	Expressed in the vasculature of the head.
TS19: head	Present	Regionally restricted	3b	Expressed in the vasculature of the head.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	3c	Expressed in neural crest cells surrounding the neuroectoderm.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	3d	Expressed in neural crest cells surrounding the entire brain, including the telencephalic vesicles. Expression is most prominent in the areas around the optic stalks and Rathke's pouch and blood vessels of the hindbrain.
TS17: blood vessel	Present	Regionally restricted	3d	Expressed in the vasculature of the hindbrain.
TS17: hindbrain	Present	Regionally restricted	3d	Expressed in the vasculature of the hindbrain.
TS17: trigeminal ganglion	Absent		3d

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	3e	Expressed in neural crest cells migrating anteriorly along the edges.
TS17: sclerotome	Present		3e

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	3f	Expressed in the mesenchyme between Rathke's pouch and the optic stalk.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	3g	Expressed in the mesenchyme immediately underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	3h	Expressed in the mesenchyme immediately underlying the epithelium.
TS17: nasal process mesenchyme	Present	Regionally restricted	3h	Expressed in the mesenchyme immediately underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	3i	Expressed in the ventral foregut splanchnic mesoderm, on either side of the laryngotracheal groove.

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Present		3j
TS17: lung mesenchyme	Weak		3j

Structure	Level	Pattern	Image	Note
TS17: hindgut mesenchyme	Strong		3k

Structure	Level	Pattern	Image	Note
TS20: digit mesenchyme	Present	Regionally restricted	4a	Expressed as stripes along the mesenchymal condensations of the forming digits.
TS20: genital tubercle	Present		4a
TS20: gut	Present		4a
TS20: spinal cord vascular element	Present		4a
TS20: oral region	Present	Regionally restricted	4a	Expressed around the oral cavity.
TS20: sclerotome	Weak	Regionally restricted	4a	Expressed as a segmented pattern with the periodicity of somites, in the posterior part of the embryo.
TS20: blood vessel	Present	Regionally restricted	4a	Expressed in the vasculature of the brain and spinal cord.

Structure	Level	Pattern	Image	Note
TS20: digit mesenchyme	Present	Regionally restricted	4b	Expressed as stripes along the mesenchymal condensations of the forming digits.
TS20: genital tubercle	Present		4b
TS20: gut	Present		4b
TS20: spinal cord vascular element	Present		4b
TS20: oral region	Present	Regionally restricted	4b	Expressed around the oral cavity.
TS20: sclerotome	Present	Regionally restricted	4b	Expression narrows anteriorly.
TS20: migrating neural crest	Weak	Regionally restricted	4b	Expressed as a segmented pattern with the periodicity of somites, in the posterior part of the embryo.
TS20: blood vessel	Present	Regionally restricted	4b	Expressed in the vasculature of the brain and spinal cord.

Structure	Level	Pattern	Image	Note
TS20: perioptic mesenchyme	Present	Clusters	4c
TS20: migrating neural crest	Present	Clusters	4c	Expressed in the neural crest cells around the eyes, cochleas, and spinal cord.

Structure	Level	Pattern	Image	Note
TS20: perioptic mesenchyme	Present		4d

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Regionally restricted	4e	Expressed in the central part. Retained only in primordia of the intervertebral discs.

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Regionally restricted	4f	Expressed in the central part. Retained only in primordia of the intervertebral discs.

Structure	Level	Pattern	Image	Note
TS20: gut mesenchyme	Present	Graded	4g	Expressed in the inner mesenchyme, closest to the epithelium. High in the central mesenchyme adjacent to the epithelium and vanishing in the periphery.

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present	Regionally restricted	4h	Expression is strongest in the mesenchyme underlying the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	4i	Expressed in the cap area of uncondensed mesenchyme at the ventral end.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	4j	Expressed in the cap area of uncondensed mesenchyme at the ventral end.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2	Present	Regionally restricted	4k	Expressed in the center of the digit primordia.
TS20: forelimb digit 3	Present	Regionally restricted	4k	Expressed in the center of the digit primordia.
TS20: forelimb digit 4	Present	Regionally restricted	4k	Expressed in the center of the digit primordia.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2 mesenchyme	Present	Regionally restricted	4l	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.
TS20: forelimb digit 3 mesenchyme	Present	Regionally restricted	4l	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	4l	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2 mesenchyme	Present	Regionally restricted	4m	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.
TS20: forelimb digit 3 mesenchyme	Present	Regionally restricted	4m	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	4m	Expressed in the mesenchyme immediately underneath the dorsal surface ectoderm.