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Gene Expression Data
Assay Details
Assay
Reference: J:199961 Lai SC, et al., The transcobalamin receptor knockout mouse: a model for vitamin B12 deficiency in the central nervous system. FASEB J. 2013 Jun;27(6):2468-75
Assay type: RT-PCR
MGI Accession ID: MGI:5529494
Gene symbol: Lrp2
Gene name: low density lipoprotein receptor-related protein 2
Probe: Megalin-pA, Megalin-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative mRNA levels were normalized to the expression in liver.
Results Image: 1E
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
Liver +/+ P m 4-6 TS28: liver Ambiguous 1 µg; total RNA Not Specified Not Specified
Liver -/- P m 4-6 TS28: liver Ambiguous 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 Cd320Gt(CC0426)Wtsi/Cd320Gt(CC0426)WtsiNot Specified
Kidney +/+ P m 4-6 TS28: metanephros Strong 1 µg; total RNA Not Specified Not Specified
Kidney -/- P m 4-6 TS28: metanephros Strong 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 Cd320Gt(CC0426)Wtsi/Cd320Gt(CC0426)WtsiNot Specified
Brain +/+ P m 4-6 TS28: brain Ambiguous 1 µg; total RNA Not Specified Not Specified
Brain -/- P m 4-6 TS28: brain Ambiguous 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 Cd320Gt(CC0426)Wtsi/Cd320Gt(CC0426)WtsiNot Specified
Spleen +/+ P m 4-6 TS28: spleen Ambiguous 1 µg; total RNA Not Specified Not Specified
Spleen -/- P m 4-6 TS28: spleen Ambiguous 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 Cd320Gt(CC0426)Wtsi/Cd320Gt(CC0426)WtsiNot Specified
Notes:
(a) Expression was similar in both wild type and knock out mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory