Antibody:	anti-phospho-SMAD2
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Structure	Level	Pattern	Image	Note
TS21: nasal cavity epithelium	Present	Regionally restricted	2A	Expressed in palate but limited to the distal portion of nasal epithelium.
TS21: palatal shelf epithelium	Present	Regionally restricted	2A	Expressed in palate but limited to the distal portion of nasal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Scattered	2B	Expression appears dispersed in the palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	2C	Expression appears concentrated at the tip of palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Present	Regionally restricted	2D	When initial contact of the opposing palatal shelves starts, expression is mainly localized in the murine medial edge epithelium, as well as in oral/nasal epithelium adjacent to the midline.
TS22: oral epithelium	Present	Regionally restricted	2D	When initial contact of the opposing palatal shelves starts, expression is mainly localized in the murine medial edge epithelium, as well as in oral/nasal epithelium adjacent to the midline.
TS22: palatal shelf epithelium	Present	Regionally restricted	2D	When initial contact of the opposing palatal shelves starts, expression is mainly localized in the murine medial edge epithelium, as well as in oral/nasal epithelium adjacent to the midline.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Present	Regionally restricted	2E	As palate fusion progresses, the expression in the midline is discontinuous and accompanied by the intense staining concentrated in the oral and nasal epithelial triangle areas.
TS22: oral epithelium	Present	Regionally restricted	2E	As palate fusion progresses, the expression in the midline is discontinuous and accompanied by the intense staining concentrated in the oral and nasal epithelial triangle areas.
TS22: primary palate epithelium	Present	Regionally restricted	2E	As palate fusion progresses, the expression in the midline is discontinuous and accompanied by the intense staining concentrated in the oral and nasal epithelial triangle areas.
TS22: primary palate mesenchyme	Present	Scattered	2E	No positive staining is found in palatal mesenchyme immediately underlying epithelia except in the mesenchymal-like cell.

Structure	Level	Pattern	Image	Note
TS23: primary palate	Absent		2F	By late E15, after palate fusion and mesenchyme confluence, no expression is detected.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Absent		2G
TS22: palatal shelf mesenchyme	Present	Regionally restricted	2G	Expressed in the sites of blood vessel formation in the distal palatal mesenchyme.
TS22: tongue muscle	Present		2G

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Absent		2H
TS22: palatal shelf mesenchyme	Present	Regionally restricted	2H	Expressed in the sites of blood vessel formation in the distal palatal mesenchyme.
TS22: tongue muscle	Present		2H

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Present		2I
TS22: tongue muscle	Present		2I

Structure	Level	Pattern	Image	Note
TS20: palatal shelf	Absent

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present			Same as the Swiss-Webster strain with regard to the distribution pattern.