Antibody:	anti-phospholipase Cbeta4
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	2A	In the anterior zone (lobules I-V), expressed in a pattern comprising strongly immunoreactive stripes, weakly reactive stripes, and negatively stained stripes.
TS27: cerebellum vermis lobule	Present	Regionally restricted	2A	In the anterior zone (lobules I-V), expressed in a pattern comprising strongly immunoreactive stripes, weakly reactive stripes, and negatively stained stripes.
TS27: cerebellar hemisphere	Present	Regionally restricted	2A	Expressed in symmetrical stripe array in the lobulus simplex and crus 2 of the ansiform lobule.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	2B	The anterior zone (lobules I-V) of the vermis interdigitates in lobule VIa with the uniformly immunonegative central zone (lobules VI-VII).
TS27: cerebellum vermis lobule VI	Present	Regionally restricted	2B	The anterior zone (lobules I-V) of the vermis interdigitates in lobule VIa with the uniformly immunonegative central zone (lobules VI-VII).
TS27: cerebellar hemisphere	Present	Regionally restricted	2B	Expressed as stripes in crus 2. No expression in crus 1.
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	2B	Expressed as stripes.
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	2B	Expressed as stripes.
TS27: cerebellum vermis lobule VII	Absent		2B	In the dorsal view.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	2C	Expressed as a complex array of stripes in posterior view.
TS27: cerebellum vermis lobule VII	Present	Regionally restricted	2C	Expressed as a complex array of stripes in posterior view.
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	2C	Expressed as a complex array of stripes in posterior view.
TS27: cerebellar hemisphere	Strong	Regionally restricted	2C	Expressed as stripes.
TS27: cerebellum vermis lobule X	Absent		2C
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	2C	Expressed as a complex array of stripes in the dorsal lobule, with no expression in the ventral lobule.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	2D	Expressed as stripes.
TS27: cerebellum vermis lobule X	Absent		2D
TS27: cerebellum vermis lobule	Present	Regionally restricted	2D	Expressed as stripes in lobule I.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	3A	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	3B	The triplet substructure in the anterior zone (lobules I-V).

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	3D	Expressed as stripes in the crus 2. A doublet structure is apparent in some stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3F	Expressed as stripes. Transient expression (not present in the adult) was seen in the Purkinje cells intervening between stripe.
TS28: cerebellum vermis lobule IX	Present	Regionally restricted	3F	Expressed as stripes. Transient expression (not present in the adult) was seen in the Purkinje cells intervening between stripe.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	3F	Expressed as stripes. Transient expression (not present in the adult) was seen in the Purkinje cells intervening between stripe.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule	Present	Regionally restricted	3H	The P2- stripe in lobule V consists of two contiguous stripes, one strongly immunoreactive medially and the other more weakly stained, located laterally.

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	3J	A transient array of stripes in the paraflocculus and flocculus.
TS28: flocculus	Present	Regionally restricted	3J	Expressed in six stripes in the ventral flocculus and in a weak stripe more rostrally that also appears to extend into the paraflocculus.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	5A	Immunoreactivity is weak or absent in lobules I-III. Two strongly immunoreactive stripes are present either side of the midline, extending through lobules IV and V and as spurs into lobule VI.
TS27: cerebellar hemisphere	Weak	Regionally restricted	5A	Weakly immunoreactive stripes are present bilaterally.
TS27: cerebellum vermis lobule VI	Present	Regionally restricted	5A	Expressed as spurs.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	5B	Two strongly immunoreactive stripes are present either side of the midline, extending through lobules III to V and as spurs into lobule VI. A tripartite structure to P1- is clearly seen in lobules III-IV.
TS27: cerebellum vermis lobule VI	Present	Regionally restricted	5B	Expressed as spurs.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule	Present	Regionally restricted	5C	Two strongly immunoreactive stripes are present either side of the midline, extending through lobules III to V and as spurs into lobule VI. A tripartite structure to P1- is clearly seen in lobules III-IV.
TS28: cerebellum vermis lobule VI	Present	Regionally restricted	5C	Expressed as spurs.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule	Present	Regionally restricted	5D	Expressed as stripes. The substructure to P1- is difficult to discern.
TS28: cerebellum vermis lobule VI	Present	Regionally restricted	5D	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule	Present	Regionally restricted	5E	Expressed as the same as the adult array of parasagittal stripes.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule VII	Absent		6A
TS27: cerebellum vermis lobule VI	Present	Regionally restricted	6A	Expressed as stripes.
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	6A	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule VII	Absent		6B
TS27: cerebellum vermis lobule VI	Present	Regionally restricted	6B	Expressed as stripes.
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	6B	Expressed as stripes.
TS27: cerebellum vermis lobule	Present	Regionally restricted	6B	No expression in the central zone (VII). Expressed as stripes in the anterior zone (V-VI) and posterior zone (VIII).

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VII	Absent		6C
TS28: cerebellum vermis lobule VI	Present	Regionally restricted	6C	Expressed as stripes.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	6C	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VII	Present	Regionally restricted	6D	Two transient broad immunoreactive stripes are detected.
TS28: cerebellum vermis lobule VI	Present	Regionally restricted	6D	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VII	Absent		6E
TS28: cerebellum vermis lobule VI	Present	Regionally restricted	6E	Expressed as stripes.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	7A	Expressed in four narrow parasagittal stripes disposed symmetrically on either side of the midline.
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	7A	Expressed in four narrow parasagittal stripes disposed symmetrically on either side of the midline. Lower expression in the intervening territory.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule VII	Absent		7B
TS27: cerebellum vermis lobule IX	Present	Regionally restricted	7B	The array of four narrow stripes on each side of the midline is better defined than at P0.
TS27: cerebellum vermis lobule VIII	Present	Regionally restricted	7B	The array of four narrow stripes on each side of the midline is better defined than at P0.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule IX	Present	Regionally restricted	7C	Expressed in four narrow parasagittal stripes. The spaces between the P1-/P2- and P2-/P3- stripes are now transiently immunoreactive. There is never staining of the midline P1+ stripe.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	7C	Expressed in four narrow parasagittal stripes. The spaces between the P1-/P2- and P2-/P3- stripes are now transiently immunoreactive. There is never staining of the midline P1+ stripe.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	7D	The transient staining of P2+ and P3+ has entirely disappeared, leaving the same array of stripes as was detected in the neonate.

Structure	Level	Pattern	Image	Note
TS27: cerebellar hemisphere	Present	Regionally restricted	8A	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	8A	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.

Structure	Level	Pattern	Image	Note
TS27: cerebellar hemisphere	Present	Regionally restricted	8B	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted	8B	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	8C	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	8C	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	8D	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	8D	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	8E	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	8E	Expressed as stripes in crus 2 and the paramedian lobule. Both P5a- and P5b- present as doublets.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	9A	Expressed as stripes. Transient expression in some future P+ stripes.