Antibody:	anti-phospholipase Cbeta4
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		1A	Expressed in the somata.
TS26: cerebellum lateral part mantle layer	Absent		1A
TS26: cerebellum lateral part marginal layer	Absent		1A
TS26: cerebellum lateral part ventricular layer	Absent		1A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		1B	Expressed in the somata and dendritic arbors.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	1C	Strong dendritic and weak somatic staining but no axonal staining.

Structure	Level	Pattern	Image	Note
TS27: cerebellum vermis lobule	Present	Regionally restricted	3C	The anterior zone expression pattern comprises strongly immunoreactive stripes, weakly reactive stripes, and stripes of Purkinje cells without expression.

Structure	Level	Pattern	Image	Note
TS28: cerebellar hemisphere	Present	Regionally restricted	3E	Expressed as a complex, reproducible array of stripes, some of which presented as doublets.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3G	Expressed in two narrow, symmetrical pairs of stripes. Inbetween there were broad stripes of positive stained Purkinje cells, especially more caudally in lobule IX; these were immunostained in the neonate but not in the adult.
TS28: cerebellum vermis lobule IX	Present	Regionally restricted	3G	Expressed in two narrow, symmetrical pairs of stripes. Inbetween there were broad stripes of positive stained Purkinje cells, especially more caudally in lobule IX; these were immunostained in the neonate but not in the adult.
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	3G	Expressed in two narrow, symmetrical pairs of stripes. Inbetween there were broad stripes of positive stained Purkinje cells, especially more caudally in lobule IX; these were immunostained in the neonate but not in the adult.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule	Present	Regionally restricted	3I	Expressed in two contiguous stripes, one strongly immunoreactive medially and the other more weakly stained, located laterally in lobule V.

Structure	Level	Pattern	Image	Note
TS28: flocculus	Present	Regionally restricted	3K	Discrete stripes cannot be resolved, but a discrete expression domain is present ventrally.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule VIII	Present	Regionally restricted	9C	Expressed as stripes. Expressed in the same embryonic clusters that also express neurogranin.