Antibody:	anti-stereocilin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: utricle epithelium	Present	Regionally restricted	1c , 1a	Protein is detected along the kinocilium of every hair cell.

Structure	Level	Pattern	Image	Note
TS28: utricle epithelium	Present	Regionally restricted	1d , 1f	Protein is detected along the kinocilium of every hair cell and far beyond the kinocilium.

Structure	Level	Pattern	Image	Note
TS28: utricle epithelium	Present		1g

Structure	Level	Pattern	Image	Note
TS28: utricle epithelium	Present		1h

Structure	Level	Pattern	Image	Note
TS28: semicircular duct ampulla	Present		1i

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	2a	Expression is in the kinocilium in outer hair cells.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2b	Expression is in the hair bundles of outer hair cells throughout the cochlea, and is restricted to the tallest row of stereocilia. Absent in hair bundles of inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2c	Expression is in the middle and the short row of stereocilia in three V-shaped stacks of labeling of each hair bundle.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2d	Expression is in the middle and the short row of stereocilia in three V-shaped stacks of labeling of each hair bundle.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	2e	Expression is in the middle and the short row of stereocilia in three V-shaped stacks of labeling of each hair bundle. Absent in hair bundles of inner hair cells.

Structure	Level	Pattern	Image	Note
TS27: tectorial membrane	Present	Regionally restricted	2g	Immunoreactive spots matched the positions of the kinocilia from the third row of outer hair cells on the undersurface at the basal cochlear turn.

Structure	Level	Pattern	Image	Note
TS28: tectorial membrane	Present	Regionally restricted	2h	Immunoreactive kinociliary spots and the single V-shaped stack of stereocilin immunoreactivity from the third and second rows of outer hair cells became associated with the membrane.

Structure	Level	Pattern	Image	Note
TS28: tectorial membrane	Present	Regionally restricted	2i	Three parallel rows of single stereocilin-immunoreactive V-shaped imprints, one from each outer hair cell, were present on the undersurface of the membrane.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present	Regionally restricted	3g	The labeling between stereocilia of the three rows is maintained in this mutant.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Strong	Regionally restricted	4d , 4a	Labeling is very strong and its distribution is expanded on outer hair cells compared to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Strong	Regionally restricted	4b , 4e	Labeling is very strong and its distribution is expanded on outer hair cells compared to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Strong	Regionally restricted	4f , 4c	Labeling is very strong and its distribution is expanded on outer hair cells compared to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Strong	Regionally restricted	4g , 4j	Labeling is very strong and its distribution is expanded on outer hair cells compared to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Strong	Regionally restricted	4h , 4k	Labeling is very strong and its distribution is expanded on outer hair cells compared to the wild-type.