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Gene Expression Data
Assay Details
Assay
Reference: J:187373 Plachez C, et al., Expression of nuclear factor one A and -B in the olfactory bulb. J Comp Neurol. 2012 Oct 1;520(14):3135-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5510867
Gene symbol: Nfib
Gene name: nuclear factor I/B
Antibody: Anti-NFIB
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5C/D: postnatal adult; Gad1tm1.1Tama/Gad1+ (more )
Note: Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Present 5D , 5C Expression in the mitral cell layer did not coincide with GAD67-GFP expression.

Specimen 5G/H: postnatal adult (more )
Note: Triple labeled: magenta - Nfib; green - Tubb3 (assay MGI:5510853); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Present Regionally restricted 5G , 5H Immunopositive mitral cells co-expressed TuJ1.

Specimen 5K/L: postnatal adult (more )
Note: Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb Present Regionally restricted 5K , 5L Expression did not coincide with GFAP-expressing astrocytes.

Specimen 10B: postnatal adult (more )
Note: Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Strong Regionally restricted 10B Strong expression was detected in cells lining the lateral ventricles. Some of these cells are surrounded by GFAP-positive fibers.

Specimen 10C: postnatal adult (more )
Note: Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
Structure Level Pattern Image Note
TS28: cerebral cortex subventricular zone Strong Regionally restricted 10C Strong expression was detected in cells lining the lateral ventricles. Some of these cells are surrounded by GFAP-positive fibers.

Specimen 11C/D: postnatal adult; Gad1tm1.1Tama/Gad1+ (more )
Note: Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 11D , 11C Low level expression was detected in the rostral migratory stream. Many of the cells were also GAD67-GFP-positive, but some cells did not express GFP.

Specimen 11K/L: postnatal adult (more )
Note: Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
Structure Level Pattern Image Note
TS28: rostral migratory stream Present Regionally restricted 11K , 11L Some immunopositive cells in the rostral migratory stream were GFAP-positive astrocytes.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/09/2024
MGI 6.23
The Jackson Laboratory