Gene Expression Data
Assay Details
Assay
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Reference:
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J:187373
Plachez C, et al., Expression of nuclear factor one A and -B in the olfactory bulb. J Comp Neurol. 2012 Oct 1;520(14):3135-49
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5510867
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Gene symbol:
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Nfib
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Gene name:
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nuclear factor I/B
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Antibody:
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Anti-NFIB
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
5C/D:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Specimen
5C/D:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
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Specimen
5G/H:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfib; green - Tubb3 (assay MGI:5510853); blue - DAPI.
Specimen
5G/H:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Triple labeled: magenta - Nfib; green - Tubb3 (assay MGI:5510853); blue - DAPI.
|
Specimen
5K/L:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
Specimen
5K/L:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: main olfactory bulb |
Present |
Regionally restricted |
5K
,
5L
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Expression did not coincide with GFAP-expressing astrocytes.
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Specimen
10B:
postnatal adult
(more )
Note: Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
Specimen
10B:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex subventricular zone |
Strong |
Regionally restricted |
10B
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Strong expression was detected in cells lining the lateral ventricles. Some of these cells are surrounded by GFAP-positive fibers.
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Specimen
10C:
postnatal adult
(more )
Note: Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
Specimen
10C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: magenta - Nfib; green - Gfap (assay MGI:5510854).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex subventricular zone |
Strong |
Regionally restricted |
10C
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Strong expression was detected in cells lining the lateral ventricles. Some of these cells are surrounded by GFAP-positive fibers.
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Specimen
11C/D:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Specimen
11C/D:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Note:
|
Triple labeled: magenta - Nfib; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
Present |
Regionally restricted |
11D
,
11C
|
Low level expression was detected in the rostral migratory stream. Many of the cells were also GAD67-GFP-positive, but some cells did not express GFP.
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Specimen
11K/L:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
Specimen
11K/L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: magenta - Nfib; green - Gfap (assay MGI:5510854); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
Present |
Regionally restricted |
11K
,
11L
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Some immunopositive cells in the rostral migratory stream were GFAP-positive astrocytes.
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