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Gene Expression Data
Assay Details
Assay
Reference: J:183957 Wu F, et al., Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. J Comp Neurol. 2012 Apr 1;520(5):952-69
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5509320
Gene symbol: Atoh7
Gene name: atonal bHLH transcription factor 7
Reporter: HA
Antibody: anti-HA tag (A00168)
Detection system: Secondary antibody
Assay notes: Two anti-HA antibodies were described (goat polyclonal and rabbit polyclonal). The antibody used in the assays was not specified, so the goat polyclonal has been assigned. See the rabbit polyclonal at MGI:5509310.
Results
Specimen not shown: embryonic day 11.5; Atoh7tm1.1Xmu/? (more )
Note: Also stained for Onecut1 (assay MGI:5509316). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS19: retina Present Regionally restricted There was almost complete overlap of expression with Oc1 in the neuroblast layer of the central retina.
Specimen 3A,A',A''': embryonic day 12.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - Onecut1 (assay MGI:5509316). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 3A , 3A''' , 3A' There was almost complete overlap of expression with Oc1 in the neuroblast layer of the central retina. No expression was detected in the ganglion cell layer.
Specimen 3B,B',B''': embryonic day 14.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - Onecut1 (assay MGI:5509316). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS22: retina Present Regionally restricted 3B' , 3B , 3B''' No expression was detected in the ganglion cell layer. In the neuroblast layer, the overlap with Oc1 was reduced significantly.
Specimen not shown: embryonic day 11.5; Atoh7tm1.1Xmu/? (more )
Note: Also stained for Onecut2 (assay MGI:5509317). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS19: retina Present Regionally restricted There was almost complete overlap of expression with Oc2 in the neuroblast layer of the central retina.
Specimen 4A,A',A''': embryonic day 12.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - Onecut2 (assay MGI:5509317). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 4A , 4A' , 4A''' There was almost complete overlap of expression with Oc2 in the neuroblast layer of the central retina. No expression was detected in the ganglion cell layer.
Specimen 4B,B',B''': embryonic day 14.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - Onecut2 (assay MGI:5509317). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS22: retina Present Regionally restricted 4B' , 4B''' , 4B No expression was detected in the ganglion cell layer. In the neuroblast layer, the overlap with Oc2 was very low.
Specimen 7A,A',A''': embryonic day 12.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - BrdU. This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 7A , 7A''' , 7A' Expression was detected largely in proliferating retinal progenitor cells.
Specimen 7C,C',C''': embryonic day 12.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - phospho-histone H3 (green). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS20: retina Present Regionally restricted 7C''' , 7C' , 7C Expression was detected largely in proliferating retinal progenitor cells.
Specimen 7D,D',D''': embryonic day 14.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - BrdU. This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS22: retina Present Regionally restricted 7D''' , 7D , 7D' At E14.5, expression continued to be largely in proliferating cells.
Specimen 7F,F',F''': embryonic day 14.5; Atoh7tm1.1Xmu/? (more )
Note: Double labeled: magenta - Atoh7; green - phospho-histone H3 (green). This specimen could have been either heterozygous or homozygous for Math5HA.
Structure Level Pattern Image Note
TS22: retina Present Regionally restricted 7F , 7F' , 7F''' At E14.5, expression continued to be largely in proliferating cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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