Probe:	Net cDNA
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present		2B
TS11: yolk sac mesoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2B	Expression is along decidual blood vessels, absent in inner decidual and trophoblastic cells.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		2E
TS13: yolk sac mesenchyme	Present		2E
TS13: heart	Present	Regionally restricted	2E	Expression is in the endocardium.
TS13: blood vessel	Present	Regionally restricted	2E	Expression is in the perineural and lateral vascular network.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	2H
TS20: blood vessel	Present	Regionally restricted	2H	Expression is in the perineural and choroid plexus vascular network.
TS20: head mesenchyme	Present	Regionally restricted	2H	Expression is in facial mesenchyme and is higher in the laryngeal pre-cartilagenous elements.
TS20: sclerotome	Strong		2H
TS20: spinal cord	Weak		2H

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Widespread	2K
TS22: blood vessel	Present		2K	Expression is in the vascular components of the liver, lung, or gut loops.
TS22: adrenal gland	Present		2K
TS22: metanephros	Present		2K
TS22: pituitary gland	Present		2K
TS22: spleen primordium	Present		2K
TS22: thymus primordium	Present		2K
TS22: lung	Weak		2K

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	4B	Expression is in the perineural and choroid plexus vascular network.
TS24: forebrain ventricular layer	Present		4B
TS24: pituitary gland	Present		4B
TS24: mandible	Strong		4B
TS24: nose	Strong		4B
TS24: cartilage	Strong		4B	Expression in in pre-cartilagenous areas such as the walls of the nasal cavities and mandible.

Structure	Level	Pattern	Image	Note
TS24: endocardial tissue	Present		4E
TS24: blood vessel	Present		4E	Expression is in the vascular components of the liver, lung, or gut loops.
TS24: lung	Weak		4E
TS24: rib	Present		4E	Expression is throughout precartilaginous condensations in ventral rib areas, but is restricted to the periphery of dorsal, more mature ribs.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	4G/H	Expression is in limb vessels.
TS22: future tarsus	Strong		4G/H
TS22: hindlimb digit cartilage condensation	Present		4G/H

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	4I/J	Expression is in limb vessels.
TS24: limb	Present	Regionally restricted	4I/J	Expression is in limb vessels.
TS24: hindlimb digit	Present	Regionally restricted	4I/J	Expression is in the perichondrial areas and future articulations, absent from mature cartilage and ossifying centers.
TS24: tarsus	Present	Regionally restricted	4I/J	Expression is in the perichondrial areas and future articulations, absent from mature cartilage and ossifying centers.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS22: pre-cartilage condensation	Strong