Antibody:	Gas1 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	2A	Expression is in the mantle layer.
TS21: axial skeleton	Present	Regionally restricted	2A	Expression is in the intervertebral tissues of the vertebral column.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Strong		2C
TS21: hindbrain	Present	Regionally restricted	2C	Expression is in the mantle layer.

Structure	Level	Pattern	Image	Note
TS21: axial skeleton	Present	Regionally restricted	2D	Expression is in the intervertebral tissues of the vertebral column.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		2E
TS21: endocardial tissue	Present		2E

Structure	Level	Pattern	Image	Note
TS21: lobar bronchus	Present	Regionally restricted	2F	Expression is in segmental bronchioles.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	2G	Expression is in the soft connective tissues surrounding the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Strong		5B	Expression is stronger in the cells at the boundary of the developing digits.
TS20: hindlimb digit	Absent		5B

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Strong		5C