Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO-MAML1*/EGFP)2Akar
Name:	transgene insertion 2, Aly Karsan
MGI ID:	MGI:5496022
Synonyms:	dnMAML-GFP, TetOS-dnMAML, TetOS-dnMAML-GFP
Transgene:	Tg(tetO-MAML1*/EGFP)2Akar  Location: unknown
Alliance:	Tg(tetO-MAML1*/EGFP)2Akar page

Strain of Origin:

ICR

Transgene Type:    Transgenic (Dominant negative, Inducible, Inserted expressed sequence, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Tg(tetO-MAML1*/EGFP)2Akar expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human MAML1 (9794) Maml1  (MGI:1890504)   Mutation details: The Tet-dnMAML-GFP transgene was designed with a TetOS promoter followed by a cDNA sequence encoding the dnMAML-GFP fusion protein and an SV40 polyA sequence. The TetOS promoter (Tet response element or TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. The dnMAML-GFP cDNA sequence encodes an N-terminal, 62 amino acid (amino acids 13-74), a dominant-negative mutant variant of human Mastermind-like 1 (dnMAML) fused in-frame at its C-terminus to an enhanced green fluorescent protein (EGFP). (J:183913)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:183913 Chang L, et al., Differentiation of vascular smooth muscle cells from local precursors during embryonic and adult arteriogenesis requires Notch signaling. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6993-8
All:	4 reference(s)