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Gene Expression Data
Assay Details
Assay
Reference: J:41699 Rosenberg P, et al., A potential role of R-cadherin in striated muscle formation. Dev Biol. 1997 Jul 1;187(1):55-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5495720
Gene symbol: Cdh4
Gene name: cadherin 4
Antibody: MRCD-5
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present 1A
TS19: mesonephric tubule Present 1A
TS19: neural tube Present 1A
TS19: notochord Present 1A
TS19: somite Present 1A

Specimen not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: limb Present Regionally restricted Expression in developing skeletal muscle in the limb.

Specimen 1C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: forelimb musculature Present Regionally restricted 1C During primary myogenesis, expression in cell-cell contacts of primary myoblasts, but not in myotubes.

Specimen 1D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: forelimb musculature Present Regionally restricted 1D During secondary myogenesis, exclusive expression at interface of primary myotube/secondary myoblasts.

Specimen 1E: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Regionally restricted 1E Expression in vascular smooth muscle cells.

Specimen 1H: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: lung Strong Regionally restricted 1H High levels of expression in smooth muscle cells surrounding clefts and stalks, but not leading edges, of epithelial tubules.
TS20: lung epithelium Weak 1H

Specimen 2A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: mesonephric tubule Present Regionally restricted 2A Expression in mesonephric epithelial duct and tubule cells.

Specimen 2B: embryonic day 12.5 (more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Structure Level Pattern Image Note
TS20: mesenchyme of metanephros Present Regionally restricted 2B Expression in aggregating mesenchymal cells which later form the epithelium of the metanephric tubular system.
TS20: pretubular aggregate Present 2B
TS20: metanephros Present Regionally restricted 2B Authors report co-expression with E-cadherin (Cdh1) in some tubular structures.
TS20: primitive collecting duct Absent 2B No expression in ureter bud-derived ducts.

Specimen 2C: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Structure Level Pattern Image Note
TS25: mesenchyme of metanephros Present Regionally restricted 2C Expression in aggregating mesenchymal cells which later form the epithelium of the metanephric tubular system.
TS25: pretubular aggregate Present 2C
TS25: s-shaped body Present Regionally restricted 2C Expression in proximal parts of s-shaped bodies
TS25: renal proximal tubule Present 2C Co-expression with E-cadherin (Cdh1) in proximal tubules.
TS25: renal distal tubule Absent 2C

Specimen 2D: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Structure Level Pattern Image Note
TS25: metanephros Present Regionally restricted 2D Co-expression with E-cadherin (Cdh1) in lateral cell-cell contacts, as well as basal plasma membrane regions. Diffuse expression in cytoplasm.

Specimen 2F: embryonic day 12.5 (more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Structure Level Pattern Image Note
TS20: thymus primordium Present Regionally restricted 2F Expression in epithelial cells.

Specimen 2H: embryonic day 17.5 (more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Structure Level Pattern Image Note
TS25: lung Present Regionally restricted 2H Selective expression in smooth muscle cells surrounding bronchioles, but not the distal airways.
TS25: lung epithelium Absent 2H


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory