Gene Expression Data
Assay Details
Assay
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Reference:
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J:41699
Rosenberg P, et al., A potential role of R-cadherin in striated muscle formation. Dev Biol. 1997 Jul 1;187(1):55-70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5495720
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Gene symbol:
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Cdh4
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Gene name:
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cadherin 4
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Antibody:
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MRCD-5
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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|
Results
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Specimen
1A:
embryonic day 11.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: limb |
Present |
Regionally restricted |
|
Expression in developing skeletal muscle in the limb.
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Specimen
1C:
embryonic day 15.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: forelimb musculature |
Present |
Regionally restricted |
1C
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During primary myogenesis, expression in cell-cell contacts of primary myoblasts, but not in myotubes.
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Specimen
1D:
embryonic day 17.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: forelimb musculature |
Present |
Regionally restricted |
1D
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During secondary myogenesis, exclusive expression at interface of primary myotube/secondary myoblasts.
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta |
Present |
Regionally restricted |
1E
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Expression in vascular smooth muscle cells.
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Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: lung |
Strong |
Regionally restricted |
1H
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High levels of expression in smooth muscle cells surrounding clefts and stalks, but not leading edges, of epithelial tubules.
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TS20: lung epithelium |
Weak |
|
1H
|
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: mesonephric tubule |
Present |
Regionally restricted |
2A
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Expression in mesonephric epithelial duct and tubule cells.
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Specimen
2B:
embryonic day 12.5
(more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
|
Specimen
2C:
embryonic day 17.5
(more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
|
Specimen
2D:
embryonic day 17.5
(more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: metanephros |
Present |
Regionally restricted |
2D
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Co-expression with E-cadherin (Cdh1) in lateral cell-cell contacts, as well as basal plasma membrane regions. Diffuse expression in cytoplasm.
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Specimen
2F:
embryonic day 12.5
(more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
|
Specimen
2H:
embryonic day 17.5
(more )
Note: Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
Specimen
2H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Cdh4; green - Cdh1 (assay MGI:5495723).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung |
Present |
Regionally restricted |
2H
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Selective expression in smooth muscle cells surrounding bronchioles, but not the distal airways.
|
TS25: lung epithelium |
Absent |
|
2H
|
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