Antibody:	Crip2 antibody
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Strong		2A	Expression in cardiogenic progenitor cells. Authors report robust expression in the heart tube.
TS13: arterial system	Present	Regionally restricted	2A	Expression in main aortic arteries. Specific expression in early vascular endothelial cells.

Structure	Level	Pattern	Image	Note
TS13: arterial system	Present	Regionally restricted	2A inset	Expression in main aortic arteries.

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Strong	Regionally restricted	2B	High expression in vascular endothelial cells.
TS15: cardiac muscle tissue	Strong		2B
TS15: endocardial lining	Strong		2B

Structure	Level	Pattern	Image	Note
TS17: outflow tract cardiac muscle	Present		2C	Persistent expression in myocardium and endocardium of outflow tract region.
TS17: outflow tract endocardial tube	Present		2C	Persistent expression in myocardium and endocardium of outflow tract region.

Structure	Level	Pattern	Image	Note
TS17: outflow tract cardiac jelly	Weak		2D	Drastically reduced expression in mesenchymal cells of outflow tract.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Present	Regionally restricted	2E	Expression in endocardium of atrioventricular cushion. Weak expression in atrioventricular cushion mesenchyme.
TS17: neural tube	Moderate		2E

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		2F" , 2F

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Weak		2G" , 2G	Lower level of expression than in myocardium.

Structure	Level	Pattern	Image	Note
TS19: visceral pericardium	Absent		2H , 2H"	No expression in epicardium.

Structure	Level	Pattern	Image	Note
TS21: heart atrium	Present		3A/B
TS21: heart ventricle	Present		3A/B
TS21: interventricular septum	Present		3A/B
TS21: mitral valve	Weak	Regionally restricted	3A/B	Relatively low level of expression in atrioventricular valves (mitral and tricuspid), restricted to endocardial layers.
TS21: tricuspid valve	Weak	Regionally restricted	3A/B	Relatively low level of expression in atrioventricular valves (mitral and tricuspid), restricted to endocardial layers.

Structure	Level	Pattern	Image	Note
TS23: heart atrium	Strong		3C/D
TS23: heart ventricle	Strong		3C/D
TS23: pulmonary valve	Weak	Regionally restricted	3C/D	Relatively low level of expression in pulmonary valves, restricted to endocardial layers.

Structure	Level	Pattern	Image	Note
TS19: cardinal vein	Present	Regionally restricted	4A	Expression in endothelial cells in large blood vessels.
TS19: dorsal aorta	Present	Regionally restricted	4A	Expression in endothelial cells in large blood vessels.
TS19: blood vessel	Present	Regionally restricted	4A	Expression in endothelial cells in capillaries.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted	4B , 4C	Expression in vascular endothelial cells.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	4E , 4D	Expression in vascular endothelial cells. No expression in smooth muscle layer of blood vessels.

Structure	Level	Pattern	Image	Note
TS19: lymphatic system	Present	Regionally restricted	4F , 4H	Expression in lymphatic endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present	Regionally restricted	5A	Expression in cardiomyocytes.

Structure	Level	Pattern	Image	Note
TS28: heart valve	Weak	Regionally restricted	5B	Weak expression in endocardial cells of heart valve leaflet.

Structure	Level	Pattern	Image	Note
TS28: blood vessel endothelium	Present	Regionally restricted	5C	Expression in endothelial cells of coronary vessel.
TS28: heart blood vessel	Present	Regionally restricted	5C	Expression in endothelial cells of coronary vessel.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present	Regionally restricted	5F , 5D	Expression in thin filaments of sarcomeres.

Structure	Level	Pattern	Image	Note
TS28: endocardial tissue	Present		5I , 5G