Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	B3galt4tm1e(EUCOMM)Wtsi
Name:	UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4; targeted mutation 1e, Wellcome Trust Sanger Institute
MGI ID:	MGI:5472736
Gene:	B3galt4  Location: Chr17:34168886-34170462 bp, - strand  Genetic Position: Chr17, 17.98 cM
IMPC:	B3galt4 gene page

Mutant Cell Line:	EPD0925_3_C01
Germline Transmission:	Unknown
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion     Vector: Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo   Mutation details: The Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 34170158 of Chromosome 17 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:155845, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any B3galt4 Mutation:	23 strains or lines available

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	2 reference(s)