Probe:	Nkx-5.2 probe
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: otocyst	Absent		2C

Structure	Level	Pattern	Image	Note
TS19: inner ear	Absent		2F	No expression in otic vesicle.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Absent		3B	No expression in most dorsal section. No expression in thalamic region.

Structure	Level	Pattern	Image	Note
TS22: midbrain tegmentum	Present		3C

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	3D	Expression in preoptic and mammillary areas.
TS22: pons	Present		3D

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present		3F	Broadest expression in the hypothalamus.
TS22: pons	Present		3F

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	3G	Expression restricted to the preoptic area.
TS22: medulla oblongata	Present		3G

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		3H
TS22: semicircular canal	Present		3H

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		3J
TS22: semicircular canal	Present		3J

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		3K

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4B	Expression in the preoptic area and mammillary area.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4C	Expression in preoptic and mammillary areas, and ventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4D	Expression in preoptic and mammillary areas, and ventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted	5A center	Expression in the rostral diencephalon

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	5A right	Expression confined to the periventricular preoptic nucleus and mediolateral preoptic nucleus.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	5B center	Caudal expression within dorsomedial hypothalamic nucleus,

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	5B right	Caudal expression within dorsomedial hypothalamic nucleus,

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted	5C center	Expression in dorsal part of premammillary nucleus.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	5C right	Expression in arcuate nucleus.

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present		5D center

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6B	Expression in the preoptic area and dorsomedial hypothalamic nucleus.
TS25: diencephalon	Present	Regionally restricted	6B	Expression in the dorsal part of the premammillary nucleus.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6C	Expression in the preoptic area and dorsomedial hypothalamic nucleus.
TS25: diencephalon	Present	Regionally restricted	6C	Expression in the dorsal part of the premammillary nucleus.
TS25: medulla oblongata	Present	Regionally restricted	6C	Expression in the solitary tract nucleus.