Probe:	M171EP
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4B
TS19: neural tube basal columns	Present	Regionally restricted	4B	Expressed in the motor neuron column. No expression in the dorsal spinal cord.
TS19: sympathetic ganglion	Strong		4B
TS19: trunk mesenchyme	Present	Regionally restricted	4B	Expressed in the mesenchymal cells surrounding sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	4D	Expressed in the dorsal spinal cord (afferent fibers of dorsal root ganglion).
TS21: dorsal root ganglion	Present		4D
TS21: basal columns	Absent		4D	No expression in the motor neuron column.
TS21: spinal cord floor plate	Weak		4D

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		5B
TS20: trigeminal nerve	Present		5B
TS20: telencephalon	Present	Regionally restricted	5B	Expressed in the mitral cells of the olfactory bulb.
TS20: nose	Present	Regionally restricted	5B	Expressed around the nasal cavity (trigeminal nerve, the veins and the olfactory nerve).
TS20: cranial nerve	Present	Regionally restricted	5B	Expressed in the olfactory nerve.
TS20: nasal cavity respiratory epithelium	Absent		5B
TS20: vomeronasal organ	Absent		5B

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	5D	Expressed in the mitral cell layer.
TS24: olfactory lobe	Present	Regionally restricted	5D	Expressed in the olfactory bulb. No expression in the accessory olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Absent		5F

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	6B	Expressed in the newly formed hippocampus.
TS21: neocortex	Present	Regionally restricted	6B	Expression was confined to the central part of the intermediate zone, but not in the cortical plate or the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present		6D
TS23: neocortex	Present	Regionally restricted	6D	Expression was confined to the central part of the intermediate zone, but not in the cortical plate or the ventricular zone.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present	Regionally restricted	6F	Expressed in the pyramidal cell layer. Expressed in some cells in the stratum oriens.
TS26: neocortex	Weak	Regionally restricted	6F	Expression was confined to the central part of the intermediate zone, but not in the cortical plate or the ventricular zone.
TS26: dentate gyrus	Present	Regionally restricted	6F	Expressed in the granular cell layer.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	6H	Expression was confined to the central part of the intermediate zone, but not in the cortical plate or the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	6J	Expressed in the mammillary body.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present		7B	Expressed in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS18: facial ganglion	Present		7D
TS18: glossopharyngeal ganglion	Present		7D
TS18: trigeminal ganglion	Present		7D
TS18: vagus ganglion	Present		7D
TS18: metencephalon	Present	Regionally restricted	7D	Expressed in the central nuclei of the trigeminal ganglia (V), the genicular ganglia of facial nerve (VII), the glossopharyngeal ganglia (IX), the vagus ganglia (X).
TS18: acoustic ganglion	Absent		7D

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	7F	Expressed in the trigeminal sensory and motor nuclei.

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	7H	Expressed in pontine nucleus.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present			Expressed in the motor neuron pool in the ventral spinal cord.
TS17: trigeminal ganglion	Present

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted		Expressed in neocortex region.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted		Expression was confined to the central part of the intermediate zone, but not in the cortical plate or the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present			Expressed in the retinal ganglion cells.

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Weak

Structure	Level	Pattern	Image	Note
TS28: neocortex	Absent

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Absent

Structure	Level	Pattern	Image	Note
TS27: trigeminal ganglion	Present

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Absent