Probe:	Uncx4.1 cDNA4
Probe preparation:	Antisense RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present		4A

Structure	Level	Pattern	Image	Note
TS13: somite	Strong	Regionally restricted	4C , 4B	Expressed in newly formed somites. Localized to less than half of the caudal part of the noncompartmentalized somite.

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present		6A
TS17: nephrogenic cord	Present		6A

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	8A	Expressed in two bands of cells (ventral and dorsal) through the entire anterior/posterior axis.
TS16: future hindbrain	Present	Regionally restricted	8A	Expressed in two bands of cells up to the midbrain/hindbrain boundary.
TS16: future midbrain	Present	Regionally restricted	8A	Expressed in the tegmentum and in the roof of the mesencephalon.
TS16: future brain	Present	Regionally restricted	8A	Expressed in posterior commissure.
TS16: mesonephros	Present		8A

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present
TS15: mesonephros	Present
TS15: future midbrain	Weak	Regionally restricted		Expressed in the ventral part.

Structure	Level	Pattern	Image	Note
TS16: nasal pit	Present