Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:1430
Hutchins JB, et al., Developmental distribution of platelet-derived growth factor in the mouse central nervous system. Brain Res Dev Brain Res. 1992 Jun 19;67(2):121-35
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5467301
|
|
Gene symbol:
|
Pdgfb
|
|
Gene name:
|
platelet derived growth factor, B polypeptide
|
|
Antibody:
|
anti-PDGF(AB)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
The antibody used in this assay recognizes the heterodimer.
|
|
|
Results
|
Specimen
3C:
embryonic day 12.5
(more  )
Note: transverse section
Specimen
3C:
(close  )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
transverse section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: basal columns |
Present |
Regionally restricted |
3C
,
3D
|
Expression is present in the future ventral horn.
|
Specimen
4C:
embryonic day 13.5
(more )
Note: parasagittal section
Specimen
4C:
(close )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
parasagittal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: basal columns |
Present |
Regionally restricted |
4C
|
Expression is present in the ventral spinal cord.
|
| TS21: dorsal root ganglion |
Present |
Widespread |
4C
,
4D
|
|
| TS21: spinal nerve |
Present |
|
4C
|
Expression is present in peripheral nerves coursing between the vertebrae. It is also present in dorsal roots entering the spinal cord.
|
Specimen
5C:
embryonic day 13.5
(more )
Note: parasagittal section
Specimen
5C:
(close )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
parasagittal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal ganglion |
Strong |
|
5C
|
Expressing cells show spike-like extensions. (This expression is thought to be due to the presence of the beta chain.)
|
Specimen
6A:
embryonic day 15.0
(more )
Note: parasagittal section
Specimen
6A:
(close )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 15.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
parasagittal section
|
Specimen
6B:
embryonic day 15.0
(more )
Note: parasagittal section
Specimen
6B:
(close )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 15.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
parasagittal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
Regionally restricted |
6B
|
Expression is present in a column in the ventrolateral spinal cord. Cells in both horns express.
|
| TS23: brainstem |
Present |
Regionally restricted |
6B
|
Expression is present in fibrous elements, as is illustrated in the inset.
|
Specimen
6E:
embryonic day 15.0
(more )
Specimen
6E:
(close )
|
Genetic Background:
|
either: (CBA x C57BL)F1 or ICR
|
|
Age:
|
embryonic day 15.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: trigeminal ganglion |
Present |
Single cells |
6E
|
Expression is present in only a few cells.
|
|
Analysis Tools