Probe:	Er81 probe
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS23: dental lamina	Present	Regionally restricted	5B
TS23: dental papilla	Strong		5B
TS23: dorsal root ganglion	Present		5B
TS23: jaw	Present		5B
TS23: tongue	Present		5B
TS23: trachea cartilaginous ring	Present		5B
TS23: skeletal muscle	Present	Regionally restricted	5B	Expression in neck muscles.
TS23: appendicular skeleton	Present	Regionally restricted	5B	Expression in young aggregating chondroblasts but not in cartilage matrix.
TS23: salivary gland	Present	Uniform	5B	Even expression in mesenchymal condense cells around growing epithelial buds.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Strong		6C	Expression in both the neural layer of the retina and in the lens.
TS17: optic cup	Strong		6C	Expression in both the neural layer of the retina and in the lens.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Strong		7C	Expression in the outer neuroblastic layer of the neuroretina.
TS23: lens equatorial epithelium	Strong	Regionally restricted	7C	Expression in the nuclear region of secondary lens fibers located in the equatorial region of the lens.
TS23: lens anterior epithelium	Strong		7C
TS23: optic choroid	Present		7C

Structure	Level	Pattern	Image	Note
TS23: nose	Present	Regionally restricted	8C	Homogenous expression in mesenchyme adjacent to the olfactory vesicle.
TS23: nasal capsule	Present	Regionally restricted	8C	Expression in perichondrial cells lining newly formed cartilage of nasal capsule and septum.
TS23: nasal septum	Present	Regionally restricted	8C	Expression in perichondrial cells lining newly formed cartilage of nasal capsule and septum.

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis	Present		9C
TS23: neurohypophysis infundibulum	Present		9C

Structure	Level	Pattern	Image	Note
TS23: mammary gland primordium epithelium	Strong		10B
TS23: skeletal muscle	Present	Regionally restricted	10B	Expression in axial, appendicular and body wall muscles.
TS23: appendicular skeleton	Present	Regionally restricted	10B	Expression in young aggregating chondroblasts but not in cartilage matrix.
TS23: atrium cardiac muscle	Strong	Regionally restricted	10B	High expression in the myogenic trabeculation of the atria as well as in the ventricles.
TS23: ventricle cardiac muscle	Strong	Regionally restricted	10B	High expression in the myogenic trabeculation of the atria as well as in the ventricles.
TS23: lung mesenchyme	Present		10B

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		11B
TS17: dermomyotome	Present	Regionally restricted	11B	Expression in the lateral dermomyotome and adjacent sclerotomal cells.
TS17: sclerotome	Present	Regionally restricted	11B	Expression in the lateral dermomyotome and adjacent sclerotomal cells.

Structure	Level	Pattern	Image	Note
TS23: gut mesenchyme	Present	Regionally restricted	12C	Expression in differentiating mesodermal layers surrounding the tracheal and digestive tract. Expression in the mesenchymal part of the mucosa.
TS23: gut epithelium	Present	Regionally restricted	12C	Expression in differentiating mesodermal layers surrounding the tracheal and digestive tract. Expression in the mesenchymal part of the mucosa.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Weak		13C

Structure	Level	Pattern	Image	Note
TS23: ureteral mesenchyme	Present	Regionally restricted	14B	Expression in the ureteric epithelium and surrounding mesenchymal condensation.
TS23: urothelium of ureter	Present	Regionally restricted	14B	Expression in the ureteric epithelium and surrounding mesenchymal condensation.

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Ambiguous		15C

Structure	Level	Pattern	Image	Note
TS17: lung mesenchyme	Present	Regionally restricted		Expression in mesenchyme surrounding the lung.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Present
TS21: tooth mesenchyme	Present
TS21: neural retinal epithelium	Strong	Regionally restricted		Expression in the outer neuroblastic layer of the neuroretina.
TS21: lens equatorial epithelium	Strong	Regionally restricted		Expression in the nuclear region of secondary lens fibers located in the equatorial region of the lens.
TS21: lens anterior epithelium	Strong

Structure	Level	Pattern	Image	Note
TS23: thyroid gland	Weak	Uniform		Expression in the epithelial cells of the peripheral capsule.

Structure	Level	Pattern	Image	Note
TS25: ureter	Weak	Regionally restricted		Expression in the epithelial cells of the peripheral capsule.

Structure	Level	Pattern	Image	Note
TS26: mesenchyme of metanephros	Weak	Uniform