About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5461573
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Reporter: lacZ
Antibody: anti-B-galactosidase (Cappel/ICN/MP Biomedicals)
Detection system: Secondary antibody coupled to Cy3
Assay notes: The authors described using three antibodies but did not specify which was used in the pictured assay; it is possible either MGI:3575669 or MGI:3529591 was used to generate some or all of these data.
Results
Specimen 3D: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3D Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
TS17: trunk dermomyotome Present Regionally restricted 3D Expression is detected in the ventrolateral lip.
TS17: trunk myotome Present 3D
TS17: body wall mesenchyme Present 3D Expression is present in the ventral somatopleure.
TS17: embryo mesenchyme Present Regionally restricted 3D Expression is present in the dorsal somatopleure.
Specimen 3E: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3E Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
TS17: trunk dermomyotome Present Regionally restricted 3E Expression is detected in the ventrolateral lip.
TS17: trunk myotome Present 3E
TS17: body wall mesenchyme Present 3E Expression is present in the ventral somatopleure.
TS17: embryo mesenchyme Present Regionally restricted 3E Expression is present in the dorsal somatopleure.
Specimen 3F: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS17: trunk myotome Present Widespread 3F Expression is present in the myotome and in migrating limb muscle precursors; some are shown at higher magnification in the inset.
Specimen 3G: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G Expression is present in muscle precursors.
Specimen 3G': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G' Expression is present in muscle precursors.
Specimen 3H: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H Expression is present in muscle precursors.
Specimen 3H': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H' Expression is present in muscle precursors.
Specimen 3I: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pitx2 protein (assay MGI:5461655).
Structure Level Pattern Image Note
TS20: forelimb Present 3I The endogenous protein and lacZ reporter protein co-localize.
Specimen 3I': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pitx2 protein (assay MGI:5461655).
Structure Level Pattern Image Note
TS20: forelimb Present 3I' The endogenous protein and lacZ reporter protein co-localize.
Specimen 3J: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J Expression is present in muscle precursors.
Specimen 3J': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J' Expression is present in muscle precursors.
Specimen 3K: embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb Absent 3K
TS20: ulnar nerve Absent 3K
Specimen 3K': embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb Absent 3K'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory