Probe:	IMAGE clone 820354
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary-mandibular groove	Present		3H
TS17: neural tube floor plate	Present		3H
TS17: neural tube roof plate	Present		3H
TS17: neural tube lateral wall	Present	Regionally restricted	3H	Expression is present in the ventral aspect (where the motor neurons originate from).
TS17: otocyst	Present		3H
TS17: trunk	Present	Regionally restricted	3H	There is expression at the junction of the posterior limb bud with the adjacent flank.
TS17: forelimb bud mesenchyme	Present		3H
TS17: heart atrium	Present		3H
TS17: hindlimb bud mesenchyme	Present		3H
TS17: primitive ventricle	Absent		3H

Structure	Level	Pattern	Image	Note
TS19: hindbrain floor plate	Present		4B
TS19: neural tube floor plate	Present		4B
TS19: brain floor plate	Present	Regionally restricted	4B	Expression is present in the floor plate up to the posterior (caudal) end of the midbrain.
TS19: diencephalon	Absent		4B
TS19: telencephalon	Absent		4B
TS19: hindbrain lateral wall	Present	Regionally restricted	4B	Expression is present in an area immediately adjacent to the floor plate at the level of rhombomere 4.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		4G
TS19: neural tube roof plate	Present		4G
TS19: neural tube basal columns	Present		4G	Expression is present in the motor neurons.
TS19: notochord	Absent		4G
TS19: trunk mesenchyme	Present	Regionally restricted	4G	Expression is present in mesenchymal cells surrounding the notochord.